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AssigneeFuji Photo Film Co., Ltd.
InventorsKouki Nakamura, Hiroko Inomata, Masayoshi Kojima, Yoshihiko Makino
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A method for detecting a hybrid multi-stranded nucleic acid sample nucleic acid, which comprises the step of allowing the sample nucleic acid to interact with a probe nucleic acid to form a hybrid multi-stranded nucleic acid by hybridization of the probe nucleic acid and the sample nucleic acid, which further comprises the steps of allowing two or more kinds of compounds with affinity to multi-stranded nucleic acids, each having a different luminescent characteristic, to interact with the hybrid multi-stranded nucleic acid, and then detecting luminescence generated as a result of an energy transfer between the two or more kinds of the compounds with affinity to multi-stranded nucleic acids.
A method for detecting a hybrid multi-stranded nucleic acid sample nucleic acid, which comprises the step of allowing the sample nucleic acid to interact with a probe nucleic acid to form a hybrid multi-stranded nucleic acid by hybridization of the probe nucleic acid and the sample nucleic acid, which further comprises the steps of allowing two or more kinds of compounds with affinity to multi-stranded nucleic acids, each having a different luminescent characteristic, to interact with the hybrid multi-stranded nucleic acid, and then detecting luminescence generated as a result of an energy transfer between the two or more kinds of the compounds with affinity to multi-stranded nucleic acids.
The method according to claim B, wherein the luminescence is detected in which the difference between wavelengths giving maximum values in excitation spectrum and emission spectrum is 100 nm or more.
A method for detecting a hybrid multi-stranded nucleic acid, which comprises the steps of allowing a sample nucleic acid to interact with a probe nucleic acid to form a hybrid multi-stranded nucleic acid by hybridization of the probe nucleic acid and sample nucleic acid, and allowing a compound with affinity to multi-stranded nucleic acid to interact with the hybrid multi-stranded nucleic acid and detecting luminescence generated from said compound to detect the hybrid multi-stranded nucleic acid, wherein the compound with affinity to multi-stranded nucleic acid is a luminescent compound having two or more kinds of chromophores, each having a different luminescent characteristic, and at least two or more kinds of the chromophores among said chromophores have a difference of 80 nm or more between a maximum absorption wavelength of a chromophore of a shorter wavelength end and a maximum emission wavelength of a chromophore of a longer wavelength end.
A method for detecting a hybrid multi-stranded nucleic acid, which comprises the steps of allowing a sample nucleic acid to interact with a probe nucleic acid to form a hybrid multi-stranded nucleic acid by hybridization of the probe nucleic acid and the sample nucleic acid, and allowing two or more kinds of compounds with affinity to multi-stranded nucleic acids, each of said compounds has a distinguishing ratio of higher than 1 for a multi-stranded nucleic acid relative to a single-stranded nucleic acid and, to interact with the hybrid multi-stranded nucleic acid and detecting interaction between said compounds to detect the hybrid multi-stranded nucleic acid, wherein at least one kind of the compound among said compounds has the distinguishing ratio of 5 or more, or each of at least two kinds of the compounds among said compounds has the distinguishing ratio of 3 or more.
Dependencies are inferred from the claim text.