Analysis of biological targets using a biochip comprising a fluorescent marker
AssigneesCommissariat a l'Energie Atomique, Universite Joseph Fourier De Grenoble
InventorsMarc Cuzin, Philippe Peltie, Marc Fontecave, Jean-Luc Decout, Cecile Dueymes
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Abstract
This invention relates to an analytical support comprising a plurality of oligonucleotides fixed on this support, characterized in that each of said oligonucleotides is marked by a fluorescent compound that presents a variation of fluorescence on the hybridization of each marked oligonucleotide with a complementary oligonucleotide. Such a support makes it possible to carry out an analysis of biological targets by measuring the variation of fluorescence in order to determine the hybridization of the targets with the oligonucleotides of the support.
Claims15
Analytical support comprising a plurality of oligonucleotides fixed on this support, characterized in that each of said oligonucleotides is marked by a fluorescent compound such that the fluorescence of an oligonucleotide marked by this compound is attenuated after hybridization of this marked oligonucleotide with a complementary oligonucleotide.
Control method for an analytical support comprising a plurality of oligonucleotides fixed on that support, characterized in that the fixed oligonucleotides are oligonucleotides marked by a fluorescent compound and in that the quantity of oligonucleotides fixed on the support is localized and determined by measurement of the fluorescence of the fluorescent compound.
Dependencies are inferred from the claim text.