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US 2003/0165908 A1Published application
Fluorometric assay for detecting nucleic acid cleavage
AssigneeGeorgetown University
InventorsMyun Ki Han, S. Paul Lee, Jack G. Chirikjian
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Abstract
A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.
Claims14
A method of detecting an enzyme-mediated nucleic acid cleavage reaction in a fluorometric assay comprising the steps of:
- (a) preparing a fluorescently labeled oligonucleotide containing a nucleic acid sequence recognizable by said enzyme, wherein said oligonucleotide acts as an enzyme substrate;
- (b) contacting said oligonucleotide of step a) with said enzyme in an amount sufficient to enzymatically cleave said oligonucleotide;
- and
- (c) detecting a nucleic acid cleavage reaction by detecting an increase in fluorescence.
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Dependencies are inferred from the claim text.