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AssigneeKurita Water Industries Ltd.
InventorsKanji Nakamura, Toshihiro Ueno
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A nucleic acid composed of 17-30 nucleotides, which hybridizes with the DNA of dechlorination bacteria preferentially at the chlorinated ethylene-decomposing gene, or a nucleic acid composed of 10-50 nucleotides, which hybridizes with the DNA of dechlorination bacteria preferentially in a region containing the base sequence(s) characteristic to high performance dechlorination bacteria present upstream from the chlorinated ethylene-decomposing gene tceA, to be used for detecting selectively dechlorination bacteria, in particular high performance dechlorination bacteria, by performing a PCR using the nucleic acid mentioned above as the primer and the nucleic acid(s) in the sample bacteria as the template and detecting the thereby synthesized DNA fragments, in order to use the thus identified dechlorination bacteria for an efficient treatment of earths or underground waters polluted by chlorinated ethylene and/or chlorinated ethane.
Nucleic acid which hybridizes with the DNA of dechlorination bacteria preferentially at the chlorinated ethylene-decomposing gene or at a position upstream thereof of the said DNA.
The nucleic acid as claimed in any one of the preceding claims, wherein the nucleic acid is assigned for detecting dechlorination bacteria.
A labelled probe for detecting dechlorination bacteria, comprising a nucleic acid as claimed in any one of the preceding claims, which nucleic acid is labelled by any of labelling means including radioactive elements, enzymes, fluorescent substances, antigens, antibodies and chemical substances.
Dependencies are inferred from the claim text.