Efficient methods for isolating functional G-protein coupled receptors and identifying active effectors and efficient methods to isolate proteins invovled in olfaction and efficient methods to isolate and identifying active effectors

20) A method of rapidly detecting proteins that interact with species-specific Gα proteins comprising:

• a) providing cell clones lacking both the endogenous Gα protein and the Gα protein's associated receptor and expressing an animal-specific-tissue-specific Gα protein gene fused to a reporter gene and each of said cell clones expressing the nucleic acid molecule of a potential Gα protein-interacting protein;
• b) providing a library of test compounds;
• c) contacting the clones with one or more test compounds;
• d) detecting a signal from clones having an interaction between the expressed tissue-specific Gα protein and expressed potential Gα protein-interacting protein;
• e) isolating the expression vector from the positive clones;
• and
• f) optionally sequencing the nucleic acid molecule of the Gα protein-interacting protein,
• whereby the protein capable of interacting with the tissue-specific Gα protein is identified.

37) A method of identifying compounds that bind receptors located on or within a plasma membrane comprising:

• a) providing at least one cell expressing a Gαo hybrid protein functionally linked to an assayable ion channel that allows influx of calcium into the cell, and a target protein;
• b) providing a library of test compounds;
• c) contacting said cell with at least one test compound;
• and
• d) detecting a calcium influx, whereby a compound capable of interacting with the target protein is identified.

51) A method of determining the presence of natural or synthetic semiochemicals in situ, comprising:

• a) providing a GPCR capable of recognizing said semiochemical of interest;
• b) incorporating said GPCR into a reported cascade;
• and
• c) detecting activation of said reporter cascade,
• whereby the presence of said semiochemical is detected

58) A method of reducing a target animal's sensitivity to odors, comprising;

• a) providing a compound known to interact with OBPs of a target species;
• b) incorporating said compound into products capable of altering pest species behavior;
• and
• c) exposing said target animal to the product containing said compound.

63) A method of manufacturing compounds or devices that mask odors, comprising:

• a) providing human OBP blockers;
• b) incorporating said human OBP blockers into a solid, particulate suspension or aqueous gel;
• and
• c) adding said solid, particulate suspension or aqueous gel to a compound or device, such as deodorants, trash cans, fertilizers, cleaning compounds, sprays to neutralize cigarette, cigar and pipe smoke.

64) A method of trapping invertebrates with odorants, comprising:

• a) providing at least one OBP or GPCR agonist;
• b) incorporating said agonist into a trap that will selectively attract said invertebrate;
• and
• c) exposing said invertebrate to the trap, whereby said invertebrate is trapped.

68) A method of identifying molecules that are GPCR agonists or antagonists, comprising:

• a) providing a transgenic animal expressing the GPCR of interest;
• b) providing a library of test compounds;
• c) exposing said animal to at least one test compound;
• and
• d) observing changes in said animal's behavior, whereby the GPCR against or antagonist is identified.

76) A method of identifying compounds that bind receptors located on or within the plasma membrane comprising:

• a) providing at least one cell expressing a BFP/Gα hybrid protein functionally linked to a YFP/Gβ hybrid protein, and a target protein;
• b) providing a library of test compounds;
• c) contacting said cell with a test compound;
• and
• d) detecting FRET,
• whereby a compound capable of interacting with the target protein is identified.