Recombinant phages capable of entering host cells via specific interaction with an artificial receptor
OwnerSerge Muyldermans, Karen Silence +2inventors
InventorsSerge Muyldermans, Karen Silence, Jan Steyaert, Els Torreele
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Abstract
The invention relates to a genetically modified bacteriophage, pseudovirion or phagemid capable of entering a host cell by binding of its artificial ligand to an artificial receptor present on said host cell. The invention relates also to the use of the genetically modified bacteriophage, pseudovirion or phagemid and of the host cell to screen sequence libraries, including antibody library.
Claims29
Genetically modified bacteriophage, pseudovirion or phagemid capable of entering a host cell by binding of its artificial ligand to an artificial receptor present on said host cell.
Method for selecting artificial receptor—artificial ligand interactions, comprising:
- growing a host cell or a mixture of host cells displaying one or more artificial receptors,
- contacting said host cell or said mixture with a genetically modified bacteriophage, pseudovirion or phagemid or a mixture of genetically modified bacteriophages, pseudovirions or phagemids with one or more artificial ligands,
- selecting those cells that have been entered by one or more bacteriophages, pseudovirion of phagemid.
Genetically modified host cell, carrying a nucleotide sequence encoding an artificial receptor in conditions enabling that the artificial receptor be expressed at the surface of the host cell, said host cell being further transformed with a nucleotide sequence encoding said artificial ligand whereby said nucleotide sequence encoding the ligand entered the host cell as a consequence of the interaction between said artificial ligand and a protein sequence or region on said artificial receptor.
Dependencies are inferred from the claim text.