Processes for purifying and for detecting target double-stranded DNA sequences by triple helix interaction
AssigneeCentelion
InventorsFrancis Blanche, Beatrice Cameron
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel target double-stranded DNA sequences capable of interacting with a third strand and of forming a stable triple helix. The present invention also relates to a process for purifying a double-stranded DNA molecule, according to which a solution containing said DNA molecule is brought into contact with a third DNA strand capable of forming, by hybridization, a triple helix structure with a target double-stranded DNA sequence carried by said DNA molecule.
Claims44
A process for purifying a double-stranded DNA molecule, comprising contacting said double-stranded DNA molecule with a third DNA strand, said double-stranded DNA molecule comprising at least one target sequence of general formula I:
- 5′-(R)n-(N)t-(R′)m-3′ (I)
- wherein:
- R and R′ represent nucleotide sequences composed only of purine bases;
- n and m are integers less than 9, and the sum of n+m is greater than 5;
- N is a nucleotide sequence comprising both purine bases and pyrimidine bases;
- and
- t is an integer less than 8;
- said DNA sequence being capable of interacting with a third DNA strand so as to form a triple helix structure.
A process for detecting a double-stranded DNA, comprising contacting a solution suspected of containing said double-stranded DNA molecule with a labeled third DNA strand capable of forming, by hybridization, a triple helix with a target sequence of said double-stranded DNA, said target sequence having the following general formula I:
- 5′-(R)n-(N)t-(R′)m-3′ (I)
- wherein:
- R and R′ represent nucleotide sequences composed only of purine bases;
- n and m are integers less than 9, and the sum of n+m is greater than 5;
- N is a nucleotide sequence comprising both purine bases and pyrimidine bases;
- and
- t is an integer less than 8.
Dependencies are inferred from the claim text.