Method and system for molecular array scanner calibration
AssigneeAgilent Technologies, Inc.
InventorsJohn F. Corson, Andreas N. Dorsel, Russell A. Parker, Andre B. Chow
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Abstract
A method and system for calibrating molecular arrays to a reference molecular array, and for subsequently calibrating the molecular arrays to maintain a constant signal-intensity-to-label-concentration ratio. In the first step of the two-step calibration method, a reference array coated with the fluorophore or chromophore used to label probe molecules is employed, while in the second step of the two-step method, a reference array coated with a stable dye is employed.
Claims10
A method for calibrating a first molecular array scanner with a second, reference molecular array scanner, the method comprising:
- initially calibrating the first molecular array scanner with the second, reference molecular array scanner using a same-dye reference array;
- and
- maintaining the initial calibration using one or more stable-dye reference arrays.
A system for calibrating a number of molecular array scanners to provide a fixed signal-intensity-to-label-concentration ratio, the system comprising:
- a reference molecular array scanner;
- a same-dye reference array used to establish an initial calibration of each of the number of molecular array scanners to the reference molecular array scanner;
- and
- one or more stable-dye reference arrays used to maintain the initial calibration.
Dependencies are inferred from the claim text.