US 2003/0165863 A1Published application

NARC10 and NARC16, programmed cell death-associated molecules and uses thereof

AssigneeMillennium Pharmaceuticals, Inc.

InventorLillian Wei-Ming Chiang

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Abstract

Novel programmed cell death-like polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length programmed cell death-related polypeptides, the invention further provides isolated programmed cell death-related fusion proteins, antigenic peptides, and anti-programmed cell death-related antibodies. The invention also provides programmed cell death-related nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a programmed cell death-related gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

Claims23

Dependent claims

An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:
2;
b) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:
4;
c) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO:
2;
d) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO:
4;
e) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
1;
f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
3;
g) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO:
1, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO:
1;
h) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO:
3, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO:
3;
i) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
1, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO:
2 under stringent conditions;
j) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
3, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO:
4 under stringent conditions;
and
k) a nucleotide sequence complementary to at least one of the nucleotide sequences set forth in a), b), c), d), e), f), g), h), i), or j).

23 more elements

An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:

a) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO:
1, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:
1;
b) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO:
3, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:
3;
c) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO:
1, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO:
2 under stringent conditions;
d) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO:
3, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO:
4 under stringent conditions;
e) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO:
1, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:
2;
and
f) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO:
3, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:
4.

16 more elements

A method for detecting the presence of a nucleic acid molecule in a sample, the method comprising the steps of contacting the sample with a nucleic acid probe that hybridizes to the nucleic acid molecule under stringent conditions, and determining whether the nucleic acid probe binds to a nucleic acid molecule in the sample, wherein said nucleic acid molecule is selected from the group consisting of:

a) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:
2;
b) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:
4;
c) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO:
2;
d) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO:
4;
e) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
1;
f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
3;
g) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO:
1, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO:
1;
h) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO:
3, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO:
3;
i) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
1, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO:
2 under stringent conditions;
j) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
3, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO:
4 under stringent conditions;
and
k) a nucleotide sequence complementary to at least one of the nucleotide sequences set forth in a), b), c), d), e), f), g), h), i), or j).

23 more elements

Dependencies are inferred from the claim text.