Compositions and methods for TIGR genotyping assays
AssigneeQuest Diagnostics Investments Incorporated
InventorDoug Hui Huang
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Abstract
Methods and compositions are described for use in the rapid and simultaneous screening of one or more samples for one or more polymorphisms in the TIGR gene. The methods and compositions of the present invention can be used to rapidly determine if polymorphisms in a gene encoding the TIGR protein are present in the genome of a subject. Identifying which polymorphisms are present in an individual can permit the diagnosis or prediction of the risk of glaucoma in the subject.
Claims21
A method, comprising:
- (a) incubating a reaction mixture comprising:
- (i) a sample nucleic acid obtained from a biological sample suspected of containing a TIGR nucleic acid sequence,
- (ii) a nucleic acid polymerase,
- (iii) one or more extension primers that specifically bind to said TIGR nucleic acid sequence if present, and that, when extended by one nucleotide at the 3′ end, comprise a nucleotide indicative of one or more preselected polymorphisms in said TIGR nucleic acid sequence, and
- (iv) one or more labeled ddNTPs,
- under conditions such that, in the presence of said TIGR nucleic acid sequence, said extension primer(s) are distinctively labeled by addition of one of said labeled ddNTP(s) to the 3′-end of said detection primer, to generate a labeled nucleic acid corresponding to one of said preselected polymorphism(s);
- and
- (b) detecting a signal from said labeled nucleic acid, wherein said signal is related to a TIGR genotype present in said sample.
An oligonucleotide consisting essentially of a sequence selected from the group consisting of SEQ ID NOS:
- 1-8.
A kit, comprising:
- one or more extension primers consisting of an oligonucleotide 17-50 bases in length, comprising at the 3′ end a sequence selected from the group consisting of SEQ ID NOS:
- 1-4;
- and
- instructions for performing a method using said one or more extension primers to perform said assay.
Dependencies are inferred from the claim text.