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US 2003/0165856 A1Published application
Methods for determining organisms not requiring the separation of fixative or excess probe
AssigneeBoston Probes, Inc.
InventorsSusan Rigby, Heather P. O'Keefe, Henrik Stender
Life of this patent
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Abstract
This invention is directed to a rapid and simple method for determining organisms and/or cells in a sample. Generally, the method is directed to the use of molecular probes to selectively stain the organisms and/or cells for determination wherein growth medium, fixative reagents and/or excess molecular probes need not be separated before a determination is made.
Claims21
A method for the analysis of organisms, cells or both organisms and cells;
- said method comprising:
- a) collecting a sample of organisms or cells;
- b) adding one or more fixative agents to the sample to thereby fix the organisms, cells or both;
- c) treating the sample with one or more molecular probes, under suitable hybridization conditions, such that the organisms, cells or both react with the molecular probe in a way that produces detectable or independently detectable organisms, cells or both;
- and
- d) determining one or more of the detectable organisms or cells in the sample;
- wherein the fixative agent or agents and excess molecular probe or probes are not separated from the organisms or cells prior to making the determination.
4 more elements
A method for determining organisms, cells or both, said method comprising:
- a) treating a sample of fixed cells, organisms or both, that have been grown in a medium, with one or more detectable molecular probes, under suitable hybridization conditions, in a way that produces stained organisms, cells or both stained organisms and cells;
- and
- b) determining the stained cells, organism or both the stained organisms and cells;
- wherein said assay does not require that the medium be removed or separated from the organisms, cells or both the organisms and cells.
1 more element
Dependencies are inferred from the claim text.