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AssigneeRoche Molecular Systems, Inc.
InventorsEberhard Russman, Thomas Meier, Ranier Schmuck, Johnny Staepels, Uwe Wehnes
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This invention relates to a method for the analysis of a (at least one) target non-proteinaceous component of a mixture of non-proteinaceous and proteinaceous components derived from a biological sample using a protease from a Bacillus strain. The invention further relates to a method for the analysis of a (at least one) target nucleic acid component of a mixture of non-proteinaceous components, which comprise nucleic acids, and proteinaceous components whereby the mixture is derived from a biological sample comprising the steps of incubating the mixture with a (at least one) protease from a Bacillus strain, optionally amplifying the (at least one) target nucleic acid component, and determining or detecting the (at least one) target nucleic acid component.
A method for the analysis of a target non-proteinaceous component of a mixture of non-proteinaceous and proteinaceous components derived from a biological sample comprising the step of incubating the mixture with a protease having an amino acid sequence which is at least 80% identical to the amino acid sequence of the protease subtilisin 147 from Bacillus lentus.
A method for the analysis of a target nucleic acid component of a mixture comprising the target nucleic acid component, and a proteinaceous component whereby the mixture is derived from a biological sample, which method comprising the steps of
A kit comprising a protease having an amino acid sequence, which is at least 80% identical to the amino acid sequence of the protease subtilisin 147 from Bacillus lentus.
A composition comprising a protease which is at least 80% identical to the amino acid sequence of the protease subtilisin 147 from Bacillus lentus, in a solution 10 mM Tris acetate pH 5.5, 5 mM calcium chloride, 5 mM calcium acetate, 1 mM EDTA, and 50% (V/V) Glycerin.
Dependencies are inferred from the claim text.