Antisense imaging of gene expression of the brain in vivo
AssigneeThe Regents of the University of California Office of Technology Transfer
InventorsWilliam M. Partridge, Ruben J. Boado
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Abstract
This invention provides imaging reagents for the detection of a gene or gene expression product (e.g. mRNA) in a brain cell in vivo. Preferred reagents comprise a detectable label attached to a first nucleic acid that specifically hybridizes to the gene or to a nucleic acid transcribed from the gene. The first nucleic acid is linked to a targeting ligand that is capable of binding a receptor on a cell comprising the blood brain barrier and crossing said blood brain barrier.
Claims61
A method of imaging in vivo expression of a gene in a brain cell of a vertebrate, said method comprising:
- i) administering to said vertebrate an imaging reagent comprising a detectable label attached to a first nucleic acid that specifically hybridizes to a second nucleic acid transcribed from said gene, where said first nucleic acid is linked to a targeting ligand that binds a receptor on a cell comprising the blood brain barrier of said vertebrate and crosses said blood brain barrier;
- and
- ii) detecting the presence or quantity of a signal produced by said detectable label in said brain cell where the presence or quantity of said label indicates the presence or quantity of a nucleic acid transcribed from said gene or cDNA.
An imaging reagent for in vivo labeling of a gene or gene product, said imaging reagent comprising a detectable label attached to a first nucleic acid that specifically hybridizes to a second nucleic acid transcribed from said gene, where said first nucleic acid is linked to a targeting ligand that binds a receptor on a cell comprising the blood brain barrier of a vertebrate and crossing said blood brain barrier.
A kit for in vivo imaging of a gene or a gene product in a brain cell, said kit comprising a container containing an imaging reagent comprising a detectable label attached to a first nucleic acid that specifically hybridizes to a second nucleic acid transcribed from said gene, where said first nucleic acid is linked to a targeting ligand that binds a receptor on a cell comprising the blood brain barrier of a vertebrate and crossing said blood brain barrier.
A kit for imaging a gene or a gene product in a brain cell, said kit comprising a container a nucleic acid that specifically hybridizes to said gene or to a nucleic acid transcribed from said gene attached to an affinity tag;
- and a container containing a targeting ligand that is capable of binding a receptor on a cell comprising the blood brain barrier of a vertebrate and crossing said blood brain barrier, where said targeting ligand is attached to an affinity tag such that when said nucleic acid is contacted to said targeting ligand the nucleic acid attaches to the targeting ligand by binding of the affinity tags.
Dependencies are inferred from the claim text.