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US 2003/0165849 A1Published application
Methods and reagents for introducing a sulfhydryl group into the 5'-terminus of RNA
OwnerBiliang Zhang, Zhiyong Cui +1inventors
InventorsBiliang Zhang, Zhiyong Cui, Lei Zhang
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Abstract
Methods for synthesizing RNA molecules whose 5′-terminus comprises a thiol group are disclosed. The present invention discloses the formation of 5′-HS-PEG-GMP-RNA and 5′-GMPS-RNA which upon alkaline phosphatase treatment, independently lead to a 5′-HS-RNA molecule.
Claims8
A method of modifying the 5′-terminus of an RNA molecule, comprising:
- obtaining a nucleoside;
- reacting the nucleoside with a chemical effective to yield a thiol group onto its 5′-terminus;
- isolating the nucleoside comprising 5′-terminus thiol molecule;
- and
- subjecting the nucleoside comprising 5′-terminus thiol molecule to an RNA polymerase molecule, dsDNA and NTPs under conditions suitable for an RNA polymerization reaction.
2 more elements
Dependencies are inferred from the claim text.