47647, a novel human lipase and uses therefor
AssigneeMillennium Pharmaceuticals, Inc.
InventorsRosana Kapeller-Libermann, Miyoung Chun
Life of this patent
Estimating the term…
Abstract
The invention provides isolated nucleic acids molecules, designated 47647 nucleic acid molecules, which encode a novel human lipase enzyme. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 47647 nucleic acid molecules, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a 47647 gene has been introduced or disrupted. The invention still further provides isolated 47647 proteins, fusion proteins, antigenic peptides and anti-47647 antibodies. Diagnostic methods utilizing compositions of the invention are also provided.
Claims23
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of one of SEQ ID NOs:
- 11 and 13;
- b) a nucleic acid molecule comprising a fragment of at least 300 nucleotides of the nucleotide sequence of one of SEQ ID NOs:
- 11 and 13, the nucleotide sequence including a portion encoding at least 5 contiguous residues of an amino acid sequence corresponding to a portion of SEQ ID NO:
- 12 selected from the group consisting of residues 244-273, 359-364, and 405-489 of SEQ ID NO:
- 12;
- c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 12;
- and
- d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 12, wherein the fragment comprises at least 25 contiguous amino acids of either of SEQ ID NO:
- 12, including at least 5 contiguous residues of an amino acid sequence corresponding to a portion of SEQ ID NO:
- 12 selected from the group consisting of residues 244-273, 359-364, and 405-489 of SEQ ID NO:
- 12.
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to a nucleic acid comprising the nucleotide sequence of one of SEQ ID NOs:
- 1, 3, 11, and 13, and a complement of one of these, the nucleic acid molecule including a portion encoding at least 5 contiguous residues of an amino acid sequence corresponding to a portion of SEQ ID NO:
- 12 selected from the group consisting of residues 244-273, 359-364, and 405-489 of SEQ ID NO:
- 12;
- b) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to a nucleic acid comprising the nucleotide sequence of one of SEQ ID NOs:
- 11 and 13, and a complement of one of these;
- and
- c) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 12, wherein the fragment comprises at least 25 contiguous amino acids of SEQ ID NO:
- 12, including at least 5 contiguous residues of a portion of SEQ ID NO:
- 12 selected from the group consisting of residues 244-273, 359-364, and 405-489 of SEQ ID NO:
- 12.
A method of inhibiting the ability of a cell to cleave a lipid, the method comprising inhibiting 47647 protein activity in the cell, whereby the ability of the cell to cleave the lipid is inhibited.
Dependencies are inferred from the claim text.