Method for identifying biological binding molecules and apparatus for carrying out the method

Abstract

The invention relates to a method for identifying the function of a ligand L using chromophore-assisted laser inactivation (CALI), characterized by the stages: a) selecting a ligand binding partner (LBP) with specificity for the ligand L, b) coupling the LBP to a laser-activatable marker (tag) to form LBP-tag, where appropriate after previous modification of the LBP with the aim of more efficient binding to the marker, c) bringing the ligand L into contact with at least one LBP-tag to form an L/LBP-tag complex, and d) irradiating the L/LBP-tag complex with a laser beam, whereupon the irradiated LBP-tag selectively modifies the bound ligand, it being possible to interchange the sequence of stages b) and c). The invention also relates to an apparatus for carrying out the method according to the invention.

Dependencies are inferred from the claim text.