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AssigneesSan Diego State University Foundation, Emerald BioStructures, Inc.
InventorsAlex B. Burgin JR., Lance J. Stewart
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The invention provides a method of nonenzymatic ligation of a nucleic acid. The method consists of contacting a polynucleotide-3′ phosphorothiolate with an acceptor polynucleotide under conditions that allow formation of a phosphodiester bond between the polynucleotide-3′ phosphorothiolate and the acceptor polynucleotide. The invention also provides methods of molecular cloning. In one embodiment, the method consists of contacting an insert comprising a polynucleotide-3′ phosphorothiolate with an acceptor vector under conditions that allow formation of a phosphodiester bond between the insert and the acceptor vector to generate a vector comprising an insert polynucleotide. The invention further provides a compound consisting of a polynucleotide-3′ phosphorothiolate and a kit containing a polynucleotide-3′ phosphorothiolate. Also provided is a method of ligating a nucleic acid using a non-sequence specific topoisomerase.
A method of non-enzymatic ligation of a nucleic acid, comprising contacting a polynucleotide-3′ phosphorothiolate with an acceptor polynucleotide under conditions that allow formation of a phosphodiester bond between said polynucleotide-3′ phosphorothiolate and said acceptor polynucleotide.
A method of molecular cloning comprising, contacting an insert comprising a polynucleotide-3′ phosphorothiolate with an acceptor vector under conditions that allow formation of a phosphodiester bond between said insert and said acceptor vector to generate a vector comprising an insert polynucleotide.
A method of molecular cloning comprising, contacting a vector comprising a polynucleotide-3′ phosphorothiolate with an acceptor polynucleotide, under conditions that allow formation of a phosphodiester bond between said vector and said acceptor polynucleotide to generate a vector comprising said acceptor polynucleotide.
A kit, comprising:
A kit, comprising:
A method of ligating a nucleic acid, comprising contacting a polynucleotide-5′ phosphorothiolate with a non-sequence specific topoisomerase, or a fragment or modification thereof, and an acceptor polynucleotide under conditions that allow formation of a phosphodiester bond between said polynucleotide-5′ phosphorothiolate and said acceptor polynucleotide, with the proviso that said polynucleotide-5′ phosphorothiolate does not contain the nucleotide sequence G(C/T)CCTT (SEQ ID NO:5).
A kit, comprising:
A composition comprising,
A compound of the formula:
A compound of the formula:
Dependencies are inferred from the claim text.