Zcys6: a member of the cystatin superfamily
AssigneeZymoGenetics, Inc.
InventorsScott R. Presnell, Zeren Gao
Life of this patent
Estimating the term…
Abstract
The cystatin superfamily includes inhibitors of cysteine proteinases, which function in a protective role with regard to various pathological actions of endogenous proteinases. Zcys6 is a new member of this superfamily.
Claims20
An isolated polypeptide, comprising an amino acid sequence that is either the amino acid sequence of SEQ ID NO:2, or the amino acid sequence of amino acid residues 99 to 143 of SEQ ID NO:2.
An isolated nucleic acid molecule, wherein the nucleic acid molecule comprises a nucleotide sequence that encodes either the amino acid sequence of SEQ ID NO:2, or the amino acid sequence of amino acid residues 99 to 143 of SEQ ID NO:2.
An expression vector, comprising a nucleic acid molecule that encodes the amino acid sequence of SEQ ID NO:2, a transcription promoter, and a transcription terminator, wherein the promoter is operably linked with the nucleic acid molecule, and wherein the nucleic acid molecule is operably linked with the transcription terminator.
A method of detecting in a biological sample the presence of RNA that encodes the amino acid sequence of SEQ ID NO:2, comprising:
- (a) contacting a nucleic acid probe under hybridizing conditions with either (i) test RNA molecules isolated from the biological sample, or (ii) nucleic acid molecules synthesized from the isolated RNA molecules, wherein the nucleic acid probe consists of at least eight nucleotides to at least 20 nucleotides of either the nucleotide sequence of nucleotides 59 to 502 of SEQ ID NO:1, or the complement of the nucleotide sequence of nucleotides 59 to 502 of SEQ ID NO:1, and
- (b) detecting the formation of hybrids of the nucleic acid probe and either the test RNA molecules or the synthesized nucleic acid molecules,
- wherein the presence of the hybrids indicates the presence of RNA that encodes the amino acid sequence of SEQ ID NO:2 in the biological sample.
A method of detecting in a biological sample the presence of a polypeptide that comprises the amino acid sequence of SEQ ID NO:2, comprising:
- (a) contacting the biological sample with an antibody, or an antibody fragment, which specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, wherein the contacting is performed under conditions that allow the binding of the antibody or antibody fragment to the biological sample, and
- (b) detecting any of the bound antibody or bound antibody fragment.
Dependencies are inferred from the claim text.