Cell stress regulated human MHC class I gene
AssigneeFred Hutchinson Cancer Research Center
InventorsThomas Spies, Veronika Spies
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Abstract
The present invention provides various methods by which the use of certain MHC-related molecules can be exploited in understanding and regulating the immune response. More particularly, the present invention describes the use of molecules expressed by certain cell types as markers, reagents and targets in the diagnosis and treatment of certain disease states including GVHD, cancer and the like.
Claims101
A method for detecting a cancer cell in a sample comprising the steps of:
- (a) providing said sample;
- and
- (b) identifying MICA or MICB expression in said sample.
The method of claim 2, wherein said identifying comprises binding of MICA or MICB by a MICA- or MICB-binding agent.
A method for purifying Vδ1 γδT cells comprising the steps of:
- (a) providing a MICA or MICB polypeptide fixed to a support;
- (b) contacting said polypeptide with a starting cell population;
- and
- (c) separating said support from said starting cell population to produce a purified Vδ1 γδT cells population.
A method for enriching a cell population for Vδ1 γδT cells comprising the steps of:
- (a) providing a MICA or MICB polypeptide linked to a fluorescent or chemilluminescent label;
- (b) contacting said polypeptide with said cell population;
- and
- (c) separating cells exhibiting fluorescence or chemilluminescence.
A method of targeting a therapeutic agent to a tumor cell expressing MICA or MICB on its surface comprising the steps of:
- (a) providing a therapeutic agent having a MICA- or MICB-binding agent conjugated thereto;
- and
- (b) contacting said therapeutic agent with said tumor cell.
A method for treating cancer comprising the step of administering to a subject a therapeutic agent having a MICA- or MICB-binding agent conjugated thereto.
A method for expanding Vδ1 γδT cells in a T-cell population comprising the steps of:
- (a) contacting said T-cell population with MICA or MICB;
- and
- (b) incubating said T-cell population under conditions permitting the growth and division of T-cells.
A method of adoptive immunotherapy comprising the step of administering to a patient a population of purified Vδ1 γδT cells.
A method of increasing expression of MICA in a cell comprising providing to said cell an expression construct comprising a coding region for MICA, wherein said coding region is under the control of a promoter active in eukaryotic cells.
A method of increasing expression of MICB in a cell comprising providing to said cell an expression construct comprising a coding region for MICB, wherein said coding region is under the control of a promoter active in eukaryotic cells.
A transgenic non-human mammal expressing a human MICA polypeptide.
A transgenic non-human mammal expressing a human MICB polypeptide.
A transgenic non-human mammal expressing human MICA and MICB polypeptides.
Dependencies are inferred from the claim text.