Polynucleotide probes for detection and quantitation of candida species
AssigneeGen-Probe Incorporated
InventorsJames J. Hogan, Patricia C. Gordon
Life of this patent
Estimating the term…
Abstract
Hybridization probes and accessory oligonucleotides useful for detecting ribosomal nucleic acids from Candida albicans, Candida tropicalis, Candida dubliniensis, Candida viswanathii and Candida parapsilosis with high specificity.
Claims36
An oligonucleotide having a sequence, said sequence comprising at least 26 contiguous nucleotides contained within SEQ ID NO:
- 7 or the complement thereof, said oligonucleotide having a length of up to 100 nucleotides.
A composition for detecting the nucleic acids of a yeast that is any of C. albicans, C. tropicalis, C. dubliniensis, C. viswanathii and C. parapsilosis, said composition comprising an oligonucleotide probe having a sequence, said sequence comprising the sequence of SEQ ID NO:
- 1 or the complement thereof, said oligonucleotide probe having a length of up to 100 nucleotide bases.
A method of determining whether an organism in the genus Candida is present in a test sample, said method comprising the steps of:
- (a) providing to said test sample a probe composition comprising an oligonucleotide probe having a sequence, said sequence comprising SEQ ID NO:
- 1, said oligonucleotide probe having a length of up to 100 nucleotide bases;
- (b) hybridizing under a high stringency condition any nucleic acid that may be present in the test sample with said probe composition to form a probe:target duplex;
- and
- (c) detecting said probe:target duplex, whereby it is determined that an organism that is any of C. albicans, C. tropicalis, C. dubliniensis, C. viswanathii and C. parapsilosis is present in the test sample.
A kit for detecting the presence of nucleic acids from any of C. albicans, C. tropicalis, C. dubliniensis, C. viswanathii and C. parapsilosis in a test sample, said kit comprising:
- (a) a composition comprising a detectably labeled oligonucleotide probe having the sequence of SEQ ID NO:
- 1;
- and
- (b) at least one helper oligonucleotide.
Dependencies are inferred from the claim text.