Nucleic acid amplification and detection of mycobacterium species
AssigneesGen-Probe Incorporated, Bio Merieux
InventorsSteven T. Brentano, Markus T. Jucker, Francisco D. Delgado, Philippe Cleuziat, Marc Rodrigue
Life of this patent
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Abstract
Methods of detecting Mycobacterium species using oligonucleotides to amplify in vitro 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species in the genus Mycobacterium are disclosed. Amplification oligonucleotides for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed. Kits containing oligonucleotides useful for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed.
Claims18
A method of detecting Mycobacterium species present in a biological sample, comprising the steps of:
- providing a biological sample containing nucleic acid from at least one Mycobacterium species comprising a Mycobacterium 16S ribosomal RNA (rRNA) or DNA encoding 16S rRNA;
- amplifying the Mycobacterium 16S rRNA or DNA in an in vitro nucleic acid amplification mixture comprising at least one polymerase activity, and at least two primers having sequences selected from the group consisting of SEQ ID NO:
- 1 to SEQ ID NO:
- 34, SEQ ID NO:
- 37 and SEQ ID NO:
- 38 to produce amplified Mycobacterium nucleic acid;
- and
- detecting the amplified Mycobacterium nucleic acid by detecting a label associated with the amplified Mycobacterium nucleic acid.
A composition for amplifying in an in vitro amplification reaction a Mycobacterium 16S rRNA sequence or DNA encoding 16S rRNA, comprising one or more oligonucleotides having a sequence selected from the group consisting of SEQ ID NO:
- 1 to SEQ ID NO:
- 34, SEQ ID NO:
- 37 and SEQ ID NO:
- 38.
A kit containing one or more oligonucleotides having a sequence selected from the group consisting of SEQ ID NO:
- 1 to SEQ ID NO:
- 34, SEQ ID NO:
- 37 and SEQ ID NO:
- 38.
Dependencies are inferred from the claim text.