Detection and identification of human papillomavirus by PCR and type-specific reverse hybridization
AssigneeInnogenetics N.V.
InventorsLeen-Jan Van Doorn, Wim Quint, Bernhard Kleter, Jan Ter Schegget
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Abstract
A method for detection and/or identification of HPV present in a biological sample comprising amplification of HPV polynucleic acids and of hybridization of said amplified polynucleic acids to a number of probes whereby a short fragment of the L1 gene of HPV is amplified after which, the amplimers are contacted with probes that specifically hybridize to the said short fragment of the L1 gene of at least one HPV type and a diagnostic kit to perform said method and primers and probes used in the said method.
Claims15
Method for detection and/or identification of HPV present in a biological sample, comprising the following steps:
- (i) amplification of a polynucleic acid fragment of HPV by use of:
- a 5′-primer specifically hybridizing to the A region or B region of the genome of at least one HPV type, said A region or B region being indicated in FIG. 1, and,
- a 3′-primer specifically hybridizing to the C region of the genome of at least one HPV type, said C region being indicated in FIG. 1;
- (ii) hybridizing the amplified fragments from step (i) with at least one probe capable of specific hybridization with the D region of at least one HPV type, said D region being indicated in FIG. 1.
An isolated HPV polynucleic acid, defined by SEQ ID NO 135 to 153, or any fragment thereof, that can be used as a primer or as a probe in a method for detection and/or identification of HPV present in a sample.
Dependencies are inferred from the claim text.