Method and kit for evaluation of hiv mutations
AssigneeVisible Genetics Inc.
InventorsJames M. Dunn, Jean-Michel Lacroix
Life of this patent
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Abstract
A method for obtaining information about the allelic type of a sample of genetic material derived from an HIV-infected sample relies on the observation that using a second nested set of sequencing primers ensures the maximum potential for sequencing a particular sample. To perform the method, reagents suitable for performing the tests are suitably packaged as a kit.
Claims13
A method for determining the genetic type of HIV-1 present in a sample containing HIV-1, comprising determining the positions of the nucleotides within the protease and reverse transcriptase genes and comparing these positions to the positions of nucleotides in known genetic types, using at least one primer selected from the group consisting of SEQ ID Nos. 1, 2, 3, 4, 5, 6, 7, 8, 20, 21, 23, 24, 25, 26, 27, 31, 32 and 33.
A kit for performing sequencing on an HIV-1 gene, comprising a plurality of termination mixtures, each of said termination mixtures including one of a plurality of primer pairs, each pair flanking a different region of the HIV-1 genome, the pairs together flanking substantially all of the protease and reverse transcriptase genes, and at least one member of each pair being labeled with a detectable label.
Dependencies are inferred from the claim text.