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AssigneeKansas State University Research Foundation
InventorsSanjay Kapil, Kumar Shanmukhappa
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Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious economic losses in swine. The present invention determined that CD 151 is a susceptibility factor for PRRSV infection by transfecting a cell line which is not susceptible to PRRSV infection (BHK-21) with CD 151, which rendered the cell line susceptible. Because CD 151 can be accessed in cellular material including blood platelets and germplasm, the present invention provides a non-invasive method of screening different swine for susceptibility to PRRSV, thereby improving breeding plans. In the case of a valuable animal, results from such screening can indicate any offspring's susceptibility to PPRSV. Additionally, the viral RNA-CD 151 interaction possesses high affinity and can be used as a tool to detect anti-viral compounds which can be further improved by using combinatorial chemistry. Accordingly, anti-viral compounds that can block the viral RNA-CD 151 interaction can be developed. Advantageously, transfection of CD 151 into non-simian cell lines can confer susceptibility to PRRSV and these recombinant cell lines can be used for preparation of biologics that will avoid simian cell lines which could be a source of primate viruses in xenotransplanted organs from pigs. Finally, the present invention describes the basic mechanism by which the virus RNA enters a target cell. This novel class of proteins is termed viral RNA entry proteins and a novel class of compounds named anti-RNA Entry Proteins can be used to block the entry of viral RNA, thereby preventing viral infections.
A method of ascertaining the susceptibility to PRRS infection in an animal comprising the steps of:
A method of comparing the PRRSV infection susceptibility of an animal to a known standard of PRRSV infection susceptibility, said known standard being correlated with a CD 151 level for cellular material of known origin, comprising the steps of:
A method for determining if an animal is resistant to PRRSV infection comprising the steps of:
A method of classifying PRRSV infection resistance in an animal comprising the steps of:
A method for detecting CD 151 in a sample of cellular material comprising the steps of:
A method of preparing PRRSV vaccine stock comprising the steps of:
A transformed cell line containing DNA coding for CD 151, said cell line, prior to transformation, not containing DNA coding for CD 151.
An isolated DNA sequence having at least about 91% sequence homology with SEQ ID NO. 1.
A plasmid containing a DNA sequence having at least about 91% sequence homology with SEQ ID NO. 1.
A plasmid having the Genbank accession number AF 275666.
A vector containing a DNA sequence having at least about 91% sequence homology with SEQ ID NO. 1.
A method of rendering a cell line susceptible to PRRSV infection comprising the step of transforming said cell line using a plasmid containing CD 151 coding sequences.
A method of selecting animals for breeding comprising the steps of:
A method of modifying PRRSV production in cells which are susceptible to PRRSV infection comprising the step of transforming said cells with CD 151.
A method of blocking entry of PRRSV into cells comprising the step of blocking PRRSV viral RNA from interaction with CD 151.
A method of diagnosing PRRSV infection in a swine herd comprising the steps of:
A method of integrating CD 151 coding sequences directly into a chromosome comprising the steps of:
A modified genome expressing an altered level of CD 151 in comparison to said genome prior to modification, said modification comprising an alteration in the number of CD 151 coding sequences in said genome.
Dependencies are inferred from the claim text.