Method for determining the activity of ornithine decarboxylase and for identifying effectors of ornithine decarboxylase activity
AssigneeSanofi-Aventis Deutschland GmbH
InventorsUrsula Mangold, Ekkehard Leberer
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for determining the activity of ornithine decarboxylase and for identifying effectors of ornithine decarboxylase activity, to the effectors of ornithine decarboxylase activity, and to the use thereof as pharmaceuticals for the treatment of disorders of the polyamine level.
Claims11
A method for determining the activity of ornithine decarboxylase (ODC), in which cells which express ODC are cultivated on a polyamine-free medium in the presence and absence of putrescine, and the growth of said cells is determined.
A method for determining the activity of antizyme, in which cells which express
- a) ornithine decarboxylase (ODC) and
- b) antizyme and, where appropriate,
- c) antizyme inhibitor are cultivated on a polyamine-free medium in the presence and absence of putrescine, and the growth of said cells is determined.
A method for determining antizyme inhibitor activity, in which cells which express
- a) ornithine decarboxylase (ODC) and
- b) antizyme and
- c) antizyme inhibitor are cultivated on a polyamine-free medium in the presence and absence of putrescine, and the growth of said cells is determined.
A method for identifying effectors of ornithine decarboxylase (ODC), in which cells which express ornithine decarboxylase (ODC) are cultivated in the presence of a test substance to be investigated on a polyamine-free medium in the presence and absence of putrescine, and the growth of said cells is determined.
A method for identifying effectors of antizyme (AZ), in which cells which express
- a) ornithine decarboxylase (ODC) and
- b) antizyme and, where appropriate,
- c) antizyme inhibitor in the presence of a test substance to be investigated are cultivated on a polyamine-free medium in the presence and absence of putrescine, and the growth of said cells is determined.
A method for identifying effectors of antizyme inhibitor (AZI), in which cells which express
- a) ornithine decarboxylase (ODC),
- b) antizyme and
- c) antizyme inhibitor in the presence of a test substance to be investigated are cultivated on a polyamine-free medium in the presence and absence of putrescine, and the growth of said cells is determined.
A test kit for identifying effectors of omithine decarboxylase, antizyme or antizyme inhibitors, comprising cells which express
- a) ornithine decarboxylase (ODC) and, where appropriate,
- b) antizyme and/or
- c) antizyme inhibitor.
Dependencies are inferred from the claim text.