US 2003/0165612 A1 · Continuous direct enzymatic protein solubilization process for industrial wastes

Abstract

Continuous direct enzymatic protein solubilization process for industrial wastes. The present invention consists of a sequential enzymatic process which enables solubilizing proteins that are present in determined industrial wastes, permitting the use of by-products. The wastes used can be of plant origin (rice bran, algarroba seed, etc.) or animal origin (Procambarus clarkii or American crab). The main process comprises two steps: a first step of successive washings with acidified water and a second step of continuous enzymatic hydrolysis. Variants to the main process are disclosed without pretreatment of the wastes with acidified water. These solubilized protein wastes (peptones) have an application, among others, in the formulation of culture media, in the biosynthesis of pharmaceutical and food products, in clinical or dietetic nutrition, as additives in cosmetics and as components of organic fertilizers.

Claims19

Dependent claims

Continuous direct protein solubilization process of industrial wastes of plant origin, characterized by comprising:

a) A first phase of successive washings of the waste with acidified water, at a pH range of 4-5.5, to wash out alkaloids such as polyphenols, sugars and fibers, followed in a continuous process by:
b) A second phase of enzymatic reaction with endoproteases which attain a degree of hydrolysis ranging from 25-40%, until obtaining, on one hand, a liquid phase constituted essentially of solubilized peptones, at a concentration ranging between 60-80% of the insoluble starting protein, and which is subsequently filtered, sterilized, evaporated and concentrated into a powder by conventional means, and on the other hand, a solid phase constituted mostly of non-solubilized proteins, starches and insoluble sugars.

Dependencies are inferred from the claim text.

Continuous direct enzymatic protein solubilization process for industrial wastes

Life of this patent

Continuous direct enzymatic protein solubilization process for industrial wastes

Life of this patent

Abstract

Claims19