Modified DNA molecule, recombinant containing the same, and uses thereof
AssigneeZeon Corporation
InventorsTakashi Okuda, Shuji Saito, Kristi M. Moore, Yoshinari Tsuzaki
Life of this patent
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Abstract
There is provided a DNA molecule derived from a prokaryotic cell in which at least one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and B is serine or threonine) has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell, and since the DNA molecule has been modified at the N-glycosylation site, it produces a non-N-glycosylated protein, which thereby exhibits a high immunogenicity when, for example, it is allowed to produce, in a eukaryotic cell, an antigen protein derived from a prokaryotic cell.
Claims20
A DNA molecule derived from a prokaryotic cell in which at least one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and B is serine or threonine) has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell.
A recombinant virus that has integrated therein
- (1) a DNA molecule derived from a prokaryotic cell in which at least one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and B is serine or threonine) has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell, or
- (2) a fused DNA molecule in which a DNA encoding a signal sequence is ligated to the N-terminal end of said modified DNA molecule so that it may be expressed as a fusion protein.
A recombinant virus that has integrated therein a fused DNA molecule, wherein a DNA encoding a signal sequence that has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell has been ligated to the N-terminal end of a DNA molecule derived from a prokaryotic cell in which at least one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and B is serine or threonine) has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell, so that it may be expressed as a fusion protein.
A method of producing an modified protein or a fusion protein comprising the same, said method comprising using:
- (1) a recombinant virus that has integrated therein a DNA molecule derived from a prokaryotic cell in which at least one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and B is serine or threonine) has been modified so that no N-glycosylation occurs during the expression in a eukaryotic cell, or
- (2) a recombinant virus that has integrated therein a fused DNA molecule in which a DNA encoding a signal sequence has been ligated to the N-terminal end of said modified DNA molecule so that it may be expressed as a fusion protein,
- to express a protein encoded by said modified DNA molecule or said fused DNA molecule in a eukaryotic cell.
Dependencies are inferred from the claim text.