Adipocyte complement related protein homolog zacrp3
OwnerChristopher S. Piddington, Paul D. Bishopinventors
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Abstract
The present invention relates to polynucleotide and polypeptide molecules for zacrp3, a novel member of the family of proteins bearing a collagen-like domain and a Clq domain. The polypeptides and polynucleotides encoding them, are involved in dimerization or oligomerization and may be used in the study thereof. The present invention also includes antibodies to the zacrp3 polypeptides.
Claims48
An isolated polypeptide comprising a sequence of amino acid residues that is at least 75% identical in amino acid sequence to residues 51-246 of SEQ ID NO:2, wherein said sequence comprises:
- Gly-Xaa-Xaa or Gly-Xaa-Pro repeats forming a collagen domain, wherein Xaa is any amino acid;
- and
- a carboxyl-terminal Clq domain comprising 10 beta strands.
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide consisting of a sequence of amino acid residues that is 80% identical in amino acid sequence to amino acid residue 51 to amino acid residue 113 of SEQ ID NO:2, said polypeptide consisting of Gly-Xaa-Xaa and Gly-Xaa-Pro repeats forming a collagen domain;
- b) a polypeptide consisting of a sequence of amino acid residues that is 80% identical in amino acid sequence to amino acid residue 114 to amino acid residue 246 of SEQ ID NO:2 comprising the sequence of SEQ ID NO:5;
- and
- c) a polypeptide consisting of a sequence of amino acid residues that is 80% identical in amino acid sequence to amino acid residue 51 to 246 of SEQ ID NO:2, said polypeptide consisting of Gly-Xaa-Xaa and Gly-Xaa-Pro repeats forming a collagen domain and comprising the sequence of SEQ ID NO:5.
A fusion protein comprises a first portion and a second portion joined by a peptide bond, said first portion consisting of a polypeptide selected from the group consisting of:
- a) a polypeptide comprising a sequence of amino acid residues that is at least 75% identical in amino acid sequence to amino acid residue 51 to amino acid residue 246 of SEQ ID NO:2;
- b) a polypeptide comprising a sequence of amino acid residues as shown in SEQ ID NO:2 from amino acid residue 1 to amino acid residue 246;
- c a portion of the zacrp3 polypeptide of SEQ ID NO:2, comprising the collagen-like domain or a portion of the collagen-like domain capable of dimerization or oligomerization;
- d) a portion of the zacrp3 polypeptide of SEQ ID NO:2, comprising the Clq domain or an active portion of the Clq domain;
- or
- e) a portion of the zacrp3 polypeptide of SEQ ID NO:2 comprising of the collagen-like domain and the Clq domain;
- and
- said second portion comprising another polypeptide.
An isolated polynucleotide encoding a polypeptide comprising a sequence of amino acid residues that is at least 75% identical in amino acid sequence to residues 51-246 of SEQ ID NO:2, wherein said sequence comprises:
- Gly-Xaa-Xaa or Gly-Xaa-Pro repeats forming a collagen domain, wherein Xaa is any amino acid;
- and
- a carboxyl-terminal Clq domain comprising 10 beta strands.
An isolated polynucleotide selected from the group consisting of,
- a) a sequence of nucleotides from nucleotide 1 to nucleotide 1696 of SEQ ID NO:1;
- b) a sequence of nucleotides from nucleotide 69 to nucleotide 806 of SEQ ID NO:1;
- c) a sequence of nucleotides from nucleotide 135 to nucleotide 806 of SEQ ID NO:1;
- d) a sequence of nucleotides from nucleotide 219 to nucleotide 806 of SEQ ID NO:1;
- e) a sequence of nucleotides from nucleotide 408 to nucleotide 806 of SEQ ID NO:1;
- f) a sequence of nucleotides from nucleotide 69 to nucleotide 407 of SEQ ID NO:1;
- g) a sequence of nucleotides from nucleotide 135 to nucleotide 407 of SEQ ID NO:1;
- h) a sequence of nucleotides from nucleotide 219 to nucleotide 407 of SEQ ID NO:1;
- i) a polynucleotide encoding a polypeptide, said polypeptide consisting of a sequence of amino acid residues that is at least 75% identical to a polypeptide consisting of the amino acid sequence of residues 51 to 113 of SEQ ID NO:2;
- j) a polynucleotide encoding a polypeptide, said polypeptide consisting of a sequence of amino acid residues that is at least 75% identical to a polypeptide consisting of the amino acid sequence of residues 114 to 246 of SEQ ID NO:2;
- k) a polynucleotide encoding a polypeptide, said polypeptide consisting of a sequence of amino acid residues that is at least 75% identical to a polypeptide consisting of the amino acid sequence of residues 51 to 246 of SEQ ID NO:2;
- l) a polynucleotide encoding a polypeptide consisting of a sequence of amino acid residues that is at least 75% identical to a polypeptide consisting of the amino acid sequence of residues 23 to 113 of SEQ ID NO:2;
- m) a polynucleotide that remains hybridized following stringent wash conditions to a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:1, or the complement of SEQ ID NO:1;
- n) nucleotide sequences complementary to a), b), c), d), e), f), g), h), i), j), k), l) or m) and
- o) degenerate nucleotide sequences of i), j), k) or l).
An isolated polynucleotide encoding a fusion protein comprising a first portion and a second portion joined by a peptide bond, said first portion is selected from the group consisting of:
- a) a polypeptide comprising a sequence of amino acid residues that is at least 75% identical in amino acid sequence to amino acid residues 51 to 246 of SEQ ID NO:2;
- b) a polypeptide comprising the sequence of amino acid residues 1 to 246 of SEQ ID NO:2;
- c) a polypeptide comprising the sequence of amino acid residues 23 to 246 of SEQ ID NO:2;
- d) a polypeptide comprising the sequence of amino acid residues 23 to 113 of SEQ ID NO:2;
- e) a polypeptide comprising the sequence of amino acid residues 1 to 113 of SEQ ID NO:2;
- f) a portion of a polypeptide of SEQ ID NO:2 comprising the collagen-like domain or a portion of the collagen-like domain capable of dimerization or oligomerization;
- g) a portion of the polypeptide of SEQ ID NO:2 containing the Clq domain;
- or
- h) a portion of the polypeptide of SEQ ID NO:2 including the collagen-like domain and the Clq domain;
- and
- said second portion comprising another polypeptide.
An isolated polynucleotide consisting of the sequence of nucleotide 1 to nucleotide 738 of SEQ ID NO:10.
Dependencies are inferred from the claim text.