Immunization of dairy cattle with GapC protein against Streptococcus infection
AssigneeUniversity of Saskacthewan
InventorsAlexandra J. Bolton, Jose Perez-Casal, Michael Fontaine, Andrew A. Potter
Life of this patent
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Abstract
The GapC plasmin binding protein genes of Streptococcus dysgalactiae (S. dysgalactiae), Streptococcus agalactiae (S. agalactiae), Streptococcus uberis (S. uberis), Streptococcus parauberis (S. parauberis), and Streptococcus iniae (S. iniae) are described, as well as the recombinant production of the GapC proteins therefrom. Also described is the use of the GapC proteins from those species in vaccine compositions to prevent or treat bacterial infections in general, and mastitis in particular.
Claims57
An isolated GapC protein selected from the group consisting of:
- (a) an isolated GapC protein comprising the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 1A-1B (SEQ ID NO:4);
- (b) an isolated GapC protein comprising an amino acid sequence with at least 98% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 2A-2B (SEQ ID NO:6);
- (c) an isolated GapC protein comprising an amino acid sequence with at least 98% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 3A-3B (SEQ ID NO:8);
- (d) an isolated GapC protein comprising an amino acid sequence with at least 98% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 4A-4B (SEQ ID NO:10);
- (e) an isolated GapC protein comprising an amino acid sequence with at least 98% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 5A-5B (SEQ ID NO:
- 12);
- and
- (f) immunogenic fragments of (a), (b), (c), (d) and (e) comprising at least 5 contiguous amino acids therefrom.
A vaccine composition comprising a pharmaceutically acceptable vehicle and a GapC protein, wherein said GapC protein is selected from the group consisting of:
- (a) a GapC protein comprising an amino acid sequence with at least 80% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 1A-1B (SEQ ID NO:4);
- (b) a GapC protein comprising an amino acid sequence with at least 80% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 2A-2B (SEQ ID NO:6);
- (c) a GapC protein comprising an amino acid sequence with at least 80% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 3A-3B (SEQ ID NO:8);
- (d) a GapC protein comprising an amino acid sequence with at least 80% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 4A-4B (SEQ ID NO:10);
- (e) a GapC protein comprising an amino acid sequence with at least 80% sequence identity to the amino acid sequence shown at amino acid positions 1 to 336, inclusive, of FIGS. 5A-5B (SEQ ID NO:12);
- and
- (f) immunogenic fragments of (a), (b), (c), (d), (e) and (f), said fragments comprising at least about 5 amino acids.
A method of detecting Streptococcus antibodies in a biological sample, comprising:
- (a) reacting said biological sample with an isolated GapC protein, or an immunogenic variant or fragment thereof, under conditions that allow said Streptococcus antibodies, when present in the biological sample, to bind to said GapC protein, or an immunogenic variant or fragment thereof, to form an antibody/antigen complex;
- and
- (b) detecting the presence or absence of said complex, thereby detecting the presence or absence of Streptococcus antibodies in said sample.
A method of detecting a GapC protein in a biological sample, comprising:
- (a) reacting said sample with antibodies directed against the GapC protein under conditions which allow said antibodies to bind to said GapC protein, when present in the sample, to form an antibody/antigen complex;
- and
- (b) detecting the presence or absence of said complex, thereby detecting the presence or absence of a GapC protein in said sample.
An immunodiagnostic test kit for detecting Streptococcus infection, said test kit comprising a GapC protein, or an immunogenic variant or fragment thereof, and instructions for conducting the immunodiagnostic test.
An immunodiagnostic test kit for detecting Streptococcus infection, said test kit comprising antibodies directed against a GapC protein and instructions for conducting the immunodiagnostic test.
Dependencies are inferred from the claim text.