Attenuated forms of bovine viral diarrhea virus
AssigneePfizer Inc.
InventorsXuemei Cao, Michael G. Sheppard
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Abstract
The present invention is directed to a method of producing attenuated forms of bovine viral diarrhea (BVD) virus by mutating the Npro protease gene. The invention includes the attenuated viruses made by this method, antibodies generated using these viruses, and vaccines that can be used for immunizing cattle.
Claims36
An attenuated bovine viral diarrhea (BVD) virus, wherein said virus has a genomic nucleic acid sequence comprising the sequence of SEQ ID NO:
- 1 from nt 39 to nt 12116, or a degenerate variant thereof.
A nucleic acid molecule, comprising the sequence of SEQ ID NO:
- 1 from nt 39 to nt 12116, or a degenerate variant thereof.
A method of modifying an isolated wild type BVD viral genome so as to make it suitable for use in a vaccine, comprising mutating the genomic nucleic acid of said isolated wild type virus to inactivate the Npro protease gene.
A method of attenuating a wild type BVD virus so as to make it suitable for use in a vaccine, comprising mutating the genomic nucleic acid of said virus to inactivate the Npro protease gene.
Dependencies are inferred from the claim text.