Mixed haptenized tumor cells and extracts and methods of treating or screening for cancer
AssigneeThomas Jefferson University
InventorDavid Berd
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Abstract
This invention relates to compositions comprising multi-haptenized tumor cells and extracts thereof, methods for preparing the compositions, vaccines comprising such multi-haptenized tumor cells, and methods for treating cancer with such vaccines. In a specific embodiment, melanoma and ovarian adenocarcinoma cells are multi-haptenized, wherein the tumor cells are differentially haptenized either with a dinitrophenyl group coupled to ε-amino groups, or with a sulfanilic acid group coupled to aromatic side chains of histidine and tyrosine. A method of SA-haptenization is also provided.
Claims25
A composition comprising a mixture of a first and a second haptenized tumor cell preparation, wherein the haptenized tumor cell preparations
- a) are differentially haptenized;
- and
- b) originate from the same tumor type as the tumor type of a subject intended for treatment with the composition.
A method for preparing a composition for use in a cancer vaccine, which method comprises differentially haptenizing at least a first and a second fraction of a tumor cell preparation and mixing cells from the differentially haptenized fractions, wherein the tumor cell preparation originates from the same type of tumor as the tumor of a subject for whom the vaccine is intended.
A method of haptenizing a cells with sulfanilic acid, comprising the steps of:
- (a) contacting an aromatic group of the cell with a sulfanilic-acid-diazonium-salt in a buffered solution at a pH lower than 8.2 to initiate a haptenization reaction;
- (b) incubating the solution for less than 15 minutes, and
- (c) terminating the haptenization reaction.
Dependencies are inferred from the claim text.