Stress protein-peptide complexes as prophylactic and therapeutic vaccines against intracellular pathogens
AssigneeMount Sinai School of Medicine of New York University
InventorPramod K. Srivastava
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Abstract
Disclosed is a family of vaccines that contain stress protein-peptide complexes which when administered to a mammal are operative to initiate in the mammal a cytotoxic T cell response against cells infected with a preselected intracellular pathogen. Also disclosed are methodologies for preparing and administering vaccines containing such stress protein-peptide complexes.
Claims36
A vaccine for administration to a mammal for inducing in the mammal a cytotoxic T cell response against a preselected intracellular pathogen, the vaccine comprising:
- (a) an immunogenic stress protein-peptide complex operative to initiate in said mammal a cytotoxic T cell response against said pathogen, said complex comprising,
- a peptide that is present in a eukaryotic cell infected with said pathogen but not present in said cell when said cell is not infected with said pathogen, complexed with a stress protein;
- and
- (b) a pharmaceutically acceptable carrier.
A vaccine for administration to a mammal for inducing in said mammal resistance to infection by a preselected intracellular pathogen, the vaccine comprising:
- (a) an immunogenic stress protein-peptide complex operative to initiate in said mammal, by means of a cytotoxic T cell response in said mammal, resistance to infection by said pathogen, said complex comprising,
- a peptide that is present in a eukaryotic cell infected with said pathogen but not present in said cell when said cell is not infected with said pathogen, complexed with a stress protein;
- and
- (b) a pharmaceutically acceptable carrier.
A method of inducing in a mammal a cytotoxic T cell response against a preselected intracellular pathogen that causes disease in said mammal, the method comprising:
- administering to said mammal a vaccine comprising,
- (a) an immunogenic stress protein-peptide complex operative to initiate in said mammal a cytotoxic T cell response against said pathogen and comprising, a peptide that is present in a eukaryotic cell infected with said pathogen but not present in said cell when said cell is not infected with said pathogen, complexed with a stress protein, and
- (b) a pharmaceutically acceptable carrier,
- in an amount sufficient to elicit in said mammal a cytotoxic T cell response against said pathogen.
A method of inducing in a mammal resistance to infection by a preselected intracellular pathogen that-causes disease in said mammal, the method comprising:
- administering to said mammal a vaccine comprising,
- (a) an immunogenic stress protein-peptide complex operative to initiate in said mammal cytotoxic T cell response against said pathogen and comprising,
- a peptide that is present in a eukaryotic cell infected with said pathogen but not present in said cell when said cell is not infected with said pathogen, complexed with a stress protein, and
- (b) a pharmaceutically acceptable carrier, in an amount sufficient to induce in said mammal, by means of the cytotoxic T cell response in said mammal, resistance to infection by said pathogen.
A method for preparing a vaccine for inducing in a mammal a cytotoxic T cell response against a preselected intracellular pathogen, the method comprising:
- (a) harvesting from a eukaryotic cell infected with said pathogen an immunogenic stress protein-peptide complex comprising,
- a peptide that is present in said cell infected with said pathogen but not present in said cell when said cell is not infected with said pathogen complexed with a stress protein, said complex, when administered to said mammal, being operative at initiating in said mammal a cytotoxic T cell response against said pathogen;
- and
- (b) combining said complex with a pharmaceutically acceptable carrier.
A method for preparing a vaccine for inducing in a mammal a cytotoxic T cell response against a preselected intracellular pathogen, the method comprising:
- (a) reconstituting in vitro a peptide that is present in a eukaryotic cell infected with said pathogen but not present in said cell when said cell is not infected with said pathogen and a stress protein, thereby to generate a stress protein-peptide complex, which when administered to said mammal is operative to initiate a cytotoxic T cell response against said pathogen in said mammal;
- and
- (b) combining said complex with a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.