Inhibition of viral infection using antigen-binding proteins
AssigneeUnilever Patent Holdings B.V.
InventorsSandra Bezemer, Leon Gerardus Joseph Frenken, Johannes Joseph Wilhelmus de Haard, Adrianus Marinus Ledeboer, Cornelis Theodorus Verrips
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Abstract
A method of inhibiting viral infection using a monovalent antigen binding protein comprising a single domain binding unit capable of binding to a virus is described. Preferably the protein is a heavy chain variable domain derived from an immunoglobulin naturally devoid of light chains. Food, pharmaceutical and cosmetic products comprising such proteins are also described together with a method for selecting inhibiting proteins from a large population of mainly containing non-inhibiting, but infectious agent binding fragments.
Claims16
A method of inhibiting viral infection using a monovalent antigen binding protein comprising a single variable domain binding unit, or a functional equivalent thereof, capable of binding to a virus.
Use of a monovalent antigen binding protein comprising a single variable domain binding unit, or a functional equivalent thereof, capable of binding to a virus in inhibiting viral infection.
Use of a monovalent antigen binding protein comprising a single variable domain binding unit, or a functional equivalent thereof, capable of binding to a virus in the preparation of a medicament for inhibiting viral infection.
A food product comprising a single variable domain binding unit, or a functional equivalent thereof, capable of binding to a virus.
A pharmaceutical or cosmetic composition comprising a single variable domain binding unit, or a functional equivalent thereof, capable of binding to a virus.
A monovalent antigen binding protein capable of inhibiting viral infection comprising a heavy chain variable domain comprising an amino acid sequence as shown in SEQ. ID No. 1, 2 or 3.
Method for selecting an antigen binding protein capable of inhibiting viral infection of a host cell comprising the steps of:
- i) complexing an antigen binding protein with a target virus,
- ii) exposing the antigen binding protein-virus complex of step (i) to an excess of host cells,
- iii) removing the host cells and any associated antigen binding protein-virus complex,
- iv) capturing antigen binding protein-virus complex not taken up by the host cells in step (ii) with virus specific ligands to separate virus specific antigen binding proteins from non-binding proteins.
Method for identifying an antigen binding protein capable of inhibiting bacteriophage infection of a lactic acid bacterial cell host comprising the steps of:
- i) culturing of bacterial host cells in the presence of antigen binding protein and bacteriophage,
- ii) assaying said culture for active cell growth manifest in a change in pH of the culture growth medium.
Dependencies are inferred from the claim text.