Compositions and methods for homoconjugates of antibodies which induce growth arrest or apoptosis of tumor cells
AssigneeBoard of Regents, The University of Texas System
InventorsMaria-Ana Ghetie, Jonathan W. Uhr, Ellen S. Vitetta
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Abstract
This invention discloses monoclonal antibodies (MAbs) which have little or no signaling activity as monomers become potent anti-tumor agents when they are converted into homoconjugates. The homoconjugates exert anti-growth activity by signaling G0/G1 arrest or apoptosis, depending upon which cell surface molecule they bind. This activity is specific and does not require an Fc portion. These conjugates are potent, anti-tumor agents.
Claims52
A conjugate of two or more monoclonal antibodies, wherein the conjugate comprises a monoclonal antibody that does not comprise an Fc region.
A method of making a conjugate of two or more monoclonal antibodies, wherein the conjugate comprises a monoclonal antibody that does not comprise an Fc region, comprising:
- obtaining a first monoclonal antibody that does not comprise an Fc region;
- obtaining a second monoclonal antibody;
- and
- conjugating the first monoclonal antibody to the second monoclonal antibody.
A method of signaling an anti-neoplastic activity comprising:
- obtaining a conjugate of two or more monoclonal antibodies, wherein the conjugate comprises a monoclonal antibody that does not comprise an Fc region and wherein the conjugate comprises a monoclonal antibody that asserts anti-neoplastic activity in a conjugated form;
- and
- contacting a neoplastic cell with the conjugate.
A method of detecting the presence of a neoplastic disease comprising:
- contacting a biological sample suspected of comprising a neoplastic antigen with a conjugate comprising a monoclonal antibody;
- and
- screening for an immunological reaction.
A pharmaceutical composition comprising a conjugate comprising a monoclonal antibody and a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.