Rabies virus-specfic neutralizing human monoclonal antibodies and nucleic acids and related methods
AssigneeThomas Jefferson University
InventorsDouglas C. Hooper, Bernhard Dietzschold
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Abstract
Human monoclonal rabies virus neutralizing antibodies represent a safe and efficacious post-exposure prophylactic therapy for individuals exposed to a rabies virus. The nucleic acid and encoded amino acid sequences of the heavy and light chain immunoglobulins of human monoclonal rabies virus neutralizing antibodies, and their use, is described.
Claims12
Isolated nucleic acid molecules, comprising a heavy chain and a light chain nucleic acid sequence that encodes a heavy chain and a light chain amino acid sequence, wherein said heavy chain and light chain amino acid sequences comprise a monoclonal rabies virus neutralizing antibody that specifically binds to a rabies virus protein.
An isolated human monoclonal rabies virus neutralizing antibody, wherein said isolated human monoclonal rabies virus neutralizing antibody is derived from cDNA clones encoding the antibody heavy and light chains expressed in heterologous expression systems and purified away from deleterious contaminants.
A fused gene encoding a chimeric immunoglobulin light chain, comprising:
- a) a first DNA sequence encoding an immunogloublin light chain variable region of a monoclonal rabies virus neutralizing antibody produced by a heterhybridoma cell line;
- and
- b) a second DNA sequence encoding a human light chain constant region.
A fused gene encoding a chimeric immunoglobulin heavy chain, comprising:
- a) a first DNA sequence encoding an immunogloublin heavy chain variable region of a monoclonal rabies virus neutralizing antibody produced by a heterhybridoma cell line;
- and
- b) a second DNA sequence encoding a human heavy chain constant region.
Dependencies are inferred from the claim text.