Anti-CCR2 antibodies and methods of use therefor
AssigneeMillennium Pharmaceuticals, Inc.
InventorsGregory J. LaRosa, Walter Newman
Life of this patent
Estimating the term…
Abstract
The present invention relates to an antibody or functional fragment thereof which binds to a mammalian (e.g., human) CC-chemokine receptor 2 (CCR2) or a portion of the receptor and blocks binding of a ligand to the receptor. The invention further relates to a method of inhibiting the interaction of a cell bearing mammalian CCR2 with a ligand thereof, and to use of the antibodies and fragments in therapeutic, prophylactic and diagnostic methods.
Claims43
An antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor.
A test kit for use in detecting the presence of a mammalian CC-chemokine receptor 2 in a biological sample comprising
- a) at least one antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor;
- and
- b) one or more ancillary reagents suitable for detecting the presence of a complex between said antibody or antigen-binding fragment thereof and said mammalian CC-chemokine receptor 2 or a portion thereof.
A method of inhibiting the interaction of a cell bearing mammalian CC-chemokine receptor 2 with a ligand thereof, comprising contacting said cell with an effective amount of an antibody or antigen-binding fragment thereof which binds to mammalian CC-chemokine receptor 2 and inhibits binding of said ligand to the receptor.
A method of inhibiting HIV infection of a cell, comprising contacting a cell with an effective amount of a composition comprising an antibody or antigen-binding fragment thereof which binds to mammalian CC-chemokine receptor 2 and inhibits HIV entry into said cell.
A method of treating HIV in a patient comprising administering to the patient a composition comprising an effective amount of an antibody or antigen-binding fragment thereof which binds to mammalian CC-chemokine receptor 2 and inhibits HIV entry into said cell.
A method of detecting expression of mammalian CC-chemokine receptor 2 or portion thereof by a cell, comprising:
- a) contacting a composition comprising a cell to be tested with an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor;
- and
- b) detecting binding of said antibody or antigen-binding fragment thereof,
- wherein the binding of said antibody or antigen-binding fragment thereof indicates the presence of said receptor or portion of said receptor on said cell.
A method of detecting a mammalian CC-chemokine receptor 2 or portion of said receptor, comprising:
- a) contacting a sample to be tested with an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor under conditions appropriate for binding of said antibody or fragment thereof thereto;
- and
- b) detecting or measuring binding of said antibody or antigen-binding fragment thereof,
- wherein the binding of said antibody or antigen-binding fragment thereof to material in said sample is indicative of the presence of a mammalian CC-chemokine receptor 2 or portion of said receptor in said sample.
A method of inhibiting a function associated with binding of a chemokine to a mammalian CC-chemokine receptor 2 or a functional portion of said receptor, comprising contacting a composition comprising the receptor or portion with an effective amount of an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody inhibits binding of said chemokine to mammalian CC-chemokine receptor 2 and inhibits one or more functions associated with binding of the ligand to the receptor.
A method of detecting or identifying an agent which binds a mammalian CC-chemokine receptor 2 or ligand-binding variant thereof, comprising combining
- a) an agent to be tested;
- b) an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor;
- and
- c) a composition comprising a mammalian CC-chemokine receptor 2 or a ligand-binding variant thereof,
- under conditions suitable for binding of said antibody or antigen-binding fragment to said mammalian CC-chemokine receptor 2 or ligand-binding variant thereof, and detecting or measuring binding of said antibody or antigen-binding fragment to said mammalian CC-chemokine receptor 2 or ligand-binding variant thereof.
A method of inhibiting HIV infection in a patient, comprising administering to the patient a composition comprising an effective amount of an antibody or antigen-binding fragment thereof which binds to mammalian CC-chemokine receptor 2 and inhibits binding of HIV to the receptor.
A method of inhibiting leukocyte trafficking in a patient, comprising administering to the patient a composition comprising an effective amount of an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2 and inhibits binding of a ligand to the receptor.
A composition comprising an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor, and an optional physiologically acceptable vehicle.
An antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor with an IC50 of less than about 1.0 μg/ml.
An antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor, and wherein the antibody or antigen-binding fragment binds the receptor with an affinity of at least about 0.1×10−9 M.
A method of treating a CC-chemokine receptor 2-mediated disorder in a patient, comprising administering to the patient an effective amount of an antibody or antigen-binding fragment thereof which binds to a mammalian CC-chemokine receptor 2, wherein said antibody or antigen-binding fragment thereof inhibits binding of a ligand to the receptor.
Dependencies are inferred from the claim text.