Antithrombin protein and DNA sequences from black fly
AssigneeAuburn University
InventorsMary S. Cupp, Eddie W. Cupp
Life of this patent
Estimating the term…
Abstract
The invention is drawn to anti-thrombin proteins from the salivary glands of the species, Simulium. Methods for recombinant production of the protein as well as biomedical uses are provided.
Claims16
An isolated nucleic acid molecule encoding a polypeptide having anti-thrombin activity, wherein said polypeptide has an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:2, said nucleic acid molecule further comprising vector nucleic acid sequences.
An isolated nucleic acid molecule encoding a polypeptide having anti-thrombin activity, wherein said nucleic acid molecule hybridizes under stringent conditions to the nucleotide sequence of SEQ ID NO:1, and wherein said nucleic acid molecule is at least 75% homologous to the nucleotide sequence set forth in SEQ ID NO:1.
A method for the abrogation of clotting in blood, said method comprising combining an effective amount of an anti-thrombin protein with a volume of blood, wherein said protein is selected from the group consisting of:
- a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2;and
- b) a polypeptide comprising an amino acid sequence having 80% identity to the amino acid sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.