Loading patent…
OwnerUlrich Zimmermann, Kurt Lucas +3inventors
InventorsUlrich Zimmermann, Kurt Lucas, Ulrich Zimmermann, Kurt Lucas, Barbara Köppen
Estimating the term…
A method of modifying antigen-presenting cells is described, in which antigens of diseased cells or their cell components are transferred to the antigen-presenting cells, the antigen-presenting cells being brought into contact with the diseased cells or their cell components on a solid-phase substrate or in a suspension through introduction into a shared suspension and exertion of external forces, membrane components having antigens without cell nuclei of the diseased cells or their cell components being transferred to the antigen-presenting cells and subsequently a separation of the modified cells from the solid-phase substrate or from the suspension being performed.
A method of modifying antigen-presenting cells, in which the antigens of diseased cells are transferred to the antigen-presenting cells, characterized in that
The method according to one of the preceding claims, wherein the antigen-presenting cells are brought into contact with the diseased cells or the cell components by exerting flow forces, for example, stirring or shaking, dielectrophoretic forces, centrifugation forces, filter techniques, sedimentation, hypoosmolar shock, and/or optical forces and chemical bonds.
The method according to one of the preceding claims, wherein the suspension is produced in an isotonic solution.
The method according to one of the preceding claims, wherein the antigen-presenting cells and the diseased cells or the cell components are subjected to a field treatment.
The method according to one of the preceding claims, wherein the cell components include membrane vesicles which are produced from membrane parts of the diseased cells.
The method according to one of the preceding claims, wherein the antigen-presenting cells include dendritic cells, T-cells, B-cells, or mast cells.
The method according to one of the preceding claims, wherein the diseased cells or cell components include tumor cells, epithelial cells, stem cells, bone marrow cells, or virus envelopes.
The method according to one of the preceding claims, wherein there is short-term contact of the dendritic cells with the diseased cells or their cell components.
The method according to one of the preceding claims, wherein an endocytosis, induced by an electric field, is performed.
A cellular tumor vaccine, which contains antigen-presenting cells, which were modified according to a method according to one of the preceding claims.
A cellular tumor vaccine, which contains antigen-presenting cells, into whose cell membranes antigens or antigen-carrying membrane components of diseased cells, particularly tumor cells, are incorporated.
A device for modifying antigen-presenting cells, particularly dendritic cells, using diseased cells or their cell components, which includes:
Dependencies are inferred from the claim text.