Methods and compositions for tissue regeneration
AssigneeDFB Pharmaceuticals, Inc.
InventorsEric Rolland, Thomas Hunziker, Beatrice Mis, Christopher Rinsch
Life of this patent
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Abstract
The present invention provides the use and composition of matter of angiogenic or other growth/cytokine factors expressed by mixtures of allogeneic human cell strains or lines of various types and stages of differentiation. Also provided are unencapsulated preparations (mixed with or applied to extracellular matrix material or synthetic biocompatible substances) for the purpose of temporary application to wounds or defects in the skin or other tissues for the restoration of blood supplying connective tissue to enable organ-specific cells to reestablish organ integrity as well as to inhibit excessive scar formation.
Claims91
A kit for the preparation of a cell preparation for tissue regeneration, the kit comprising, in one or more containers
- a) a first component comprising an extracellular matrix or matrix material;
- and
- b) a second component comprising one or more cell types that secrete a biologically active molecule, wherein the cell types are allogeneic, mitotically active or inactivated, and are selected from the group consisting of stromal, epithelial/organ specific and blood-derived cells.
A method of administering a cell preparation for tissue regeneration to a wound site on a patient in need of treatment, the method comprising
- a) providing a first component comprising an extracellular matrix or matrix material containing fibrinogen;
- b) providing a second component comprising from about 1×103 cells/μl to about 50×103 cells/μl and thrombin, wherein the cells secrete one or more biologically active molecules, are allogeneic, mitotically inactivated, and selected from the group consisting of stromal, epithelia/organ specific, and blood-derived cells;
- c) combining the first and second components to form a cell preparation suitable for tissue regeneration;
- and
- d) administering the cell preparation to the wound site.
A cell preparation for tissue regeneration comprising
- a) a first component comprising an extracellular matrix or matrix material containing fibrinogen admixed with
- b) a second component comprising from about 1×103 cells/μl to about 50×103 cells/μl and thrombin to the wound site, wherein the cells secrete one or more biologically active molecules, are allogeneic, mitotically active or inactivated, and selected from the group consisting of stromal, epithelia/organ specific, and blood-derived cells.
Dependencies are inferred from the claim text.