Macroaggregated protein conjugates as oral genetic immunization delivery agents
AssigneeBaylor College of Medicine
InventorsFrank M. Orson, Berma M. Kinsey, Balbir S. Bhogal
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Abstract
This invention relates to the development of an expression vector containing an antigenic genomic sequence, which is bound to an aggregated protein-polycationic polymer conjugate. More particularly it relates to the use of the expression vector as an oral DNA vaccine or to induce immune response. In specific embodiments, the expression vector is bound to a protein-polycationic polymer suspension.
Claims57
A composition comprising an expression vector bound to an aggregated protein-polycationic polymer conjugate, wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen.
A method of producing a DNA vaccine comprising the step of incubating an expression vector with an aggregated protein-polycationic polymer conjugate to form DNA particles wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen.
A method of inducing an immune response in an organism comprising the step of administering to an organism an expression vector bound to an aggregated protein-polycationic polymer conjugate wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen.
A method of inducing an immune response in an organism comprising the step of co-administering to an organism an expression vector bound to an aggregated protein-polycationic polymer conjugate wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen and a cytokine expression vector.
A method of inducing an immune response in an organism comprising the step of administering to an organism an expression vector bound to an aggregated protein-polycationic polymer conjugate wherein the expression vector comprises a first promoter polynucleotide sequence operatively linked to a first polynucleotide sequence encoding an antigen and a second polynucleotide sequence encoding a cytokine.
A method of introducing genes into a cell comprising the steps of:
- forming a DNA particle comprising an expression vector bound to an aggregated protein-polycationic polymer conjugate wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen;
- and incubating the cells with the DNA particle under conditions wherein the cells take in the DNA particle.
A composition comprising an expression vector bound to a protein-polycationic polymer suspension, wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen.
A method of inducing an immune response in an organism comprising the step of administering to an organism an expression vector bound to a protein-polycationic polymer suspension wherein the expression vector comprises a promoter polynucleotide sequence operatively linked to a polynucleotide sequence encoding an antigen.
Dependencies are inferred from the claim text.