Urinary tract tissue graft compositions and methods for producing same

A urinary tract tissue graft composition, comprising:

• a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• smooth muscle cells seeded on the mucosal surface of the distal ileal segment of small intestinal submucosa;
• and
• urothelial cells seeded on the serosal surface of the distal ileal segment of small intestinal submucosa.

A urinary tract tissue graft composition, comprising:

• a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• smooth muscle cells seeded on the mucosal surface of the distal ileal segment of small intestinal submucosa;
• and
• urothelial cells seeded on the smooth muscle cells attached to the mucosal surface of the distal ileal segment of small intestinal submucosa.

A method for providing a urinary tract tissue graft composition, comprising:

• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• isolating and culturing at least one cell type from a tissue specimen of a subject;
• and
• seeding the at least one cell type on the distal ileal segment of small intestinal submucosa.

A method for providing a urinary tract tissue graft composition, comprising:

• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• isolating and culturing smooth muscle cells from a tissue specimen of a subject;
• isolating and culturing urothelial cells from a tissue specimen of a subject;
• seeding the smooth muscle cells on the distal ileal segment of small intestinal submucosa;
• and
• seeding the urothelial cells on the distal ileal segment of small intestinal submucosa.

A method for providing a urinary tract tissue graft composition, comprising:

• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• isolating and culturing bladder smooth muscle cells from a bladder tissue specimen of a subject;
• isolating and culturing bladder urothelial cells from a bladder tissue specimen of a subject;
• seeding the bladder smooth muscle cells on the mucosal surface of the distal ileal segment of small intestinal submucosa;
• and
• seeding the bladder urothelial cells on the serosal surface of the distal ileal segment of small intestinal submucosa.

A method for providing a urinary tract tissue graft composition, comprising:

• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• isolating and culturing bladder smooth muscle cells from a bladder tissue specimen of a subject;
• isolating and culturing bladder urothelial cells from a bladder tissue specimen of a subject;
• seeding the bladder smooth muscle cells on the mucosal surface of the distal ileal segment of small intestinal submucosa;
• and
• seeding the bladder urothelial cells on the bladder smooth muscle cells attached to the mucosal surface of the distal ileal segment of small intestinal submucosa.

A method for repairing a damaged or diseased urinary tract tissue of a subject, comprising the steps of:

• isolating and culturing smooth muscle cells from a urinary tract tissue specimen of a subject;
• isolating and culturing urothelial cells from the urinary tract tissue specimen of the subject;
• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• seeding the smooth muscle cells on the distal ileal segment of small intestinal submucosa;
• and
• seeding the urothelial cells on the distal ileal segment of small intestinal submucosa;
• allowing the distal ileal segment of small intestinal submucosa having the smooth muscle cells and urothelial cells thereon to mature in culture such that the smooth muscle cells and urothelial cells exhibit three dimensional growth and matrix penetrance;
• removing the distal ileal segment of small intestinal submucosa from the tissue culture frame;
• and
• contacting the damaged urinary tract tissue with the distal ileal segment of small intestinal submucosa under conditions such that growth of the urinary tract tissue occurs and the damaged urinary tract tissue is repaired, thereby restoring urological function.

A method for repairing a damaged urinary tract tissue of a subject, comprising the steps of:

• isolating and culturing smooth muscle cells from a urinary tract tissue specimen of a subject;
• isolating and culturing urothelial cells from the urinary tract tissue specimen of the subject;
• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• seeding smooth muscle cells on the mucosal surface of the distal ileal segment of small intestinal submucosa;
• and
• seeding urothelial cells on the serosal surface of the distal ileal segment of small intestinal submucosa;
• allowing the distal ileal segment of small intestinal submucosa having the smooth muscle cells and urothelial cells thereon to mature in culture such that the smooth muscle cells and urothelial cells exhibit three dimensional growth and matrix penetrance;
• removing the distal ileal segment of small intestinal submucosa from the tissue culture frame;
• and
• contacting the damaged urinary tract tissue with the distal ileal segment of small intestinal submucosa under conditions such that growth of the urinary tract tissue occurs and the damaged urinary tract tissue is repaired, thereby restoring urological function.

A method for repairing a damaged urinary tract tissue of a subject, comprising the steps of:

• isolating and culturing smooth muscle cells from a urinary tract tissue specimen of a subject;
• isolating and culturing urothelial cells from the urinary tract tissue specimen of the subject;
• providing a tissue culture frame;
• providing a distal ileal segment of small intestinal submucosa having a mucosal surface and a serosal surface;
• positioning the distal ileal segment of small intestinal submucosa in the tissue culture frame such that the distal ileal segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame;
• seeding smooth muscle cells on the mucosal surface of the distal ileal segment of small intestinal submucosa;
• and
• seeding urothelial cells on the smooth muscle cells attached to the mucosal surface of the distal ileal segment of small intestinal submucosa;
• allowing the distal ileal segment of small intestinal submucosa having the smooth muscle cells and urothelial cells thereon to mature in culture such that the smooth muscle cells and urothelial cells exhibit three dimensional growth and matrix penetrance;
• removing the distal ileal segment of small intestinal submucosa from the tissue culture frame;
• and
• contacting the damaged urinary tract tissue with the small intestinal submucosa under conditions such that growth of the urinary tract tissue occurs and the damaged urinary tract tissue is repaired, thereby restoring urological function.