Engineered intervertebral disc tissue
AssigneeRush-Presbyterian-St. Luke's Medical Center
InventorsKoichi Masuda, Howard S. An, Eugene J-M. A. Thonar
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Abstract
Surgically implantable tissue engineered intervertebral disc tissues that effectively replicate the physicochemical properties of the corresponding in vivo tissues are provided. Methods for producing such tissues can involve culturing the intervertebral disc cells to produce cells surrounded by an extracellular matrix and culturing the cells and matrix on a semipermeable membrane to form a cohesive tissue.
Claims20
A method for producing an engineered intervertebral disc tissue, comprising:
- (a) culturing intervertebral disc cells in a medium for an effective amount of time to produce intervertebral disc cells surrounded by a cell-associated matrix;
- and
- (b) culturing the intervertebral disc cells surrounded by the cell-associated on a semipermeable membrane in the presence of one or more growth factors for a sufficient amount of time to produce a coherent, engineered intervertebral disc tissue.
A cohesive engineered intervertebral disc tissue comprised of greater than or about 80 percent water by weight, between at or about 0.95 and 7.5 μg/mg DNA, between at or about 100 and 350 μg/mg proteoglycan, and between at or about 75 and 450 μg/mg collagen, wherein the DNA, proteoglycan and collagen amounts are based on the dry weight of the engineered tissue.
A method for surgically repairing intervertebral disc damage, comprising:
- (a) producing a transplantable intervertebral disc tissue in vitro;
- and
- (b) implanting the intervertebral disc tissue into an intervertebral disc defect.
A kit for producing an intervertebral disc tissue comprising:
- (a) instructions for producing an intervertebral disc tissue;
- and one or more:
- (b) growth media;
- (c) semipermeable membranes;
- (d) growth factors;
- (e) one or more pieces of disposable lab equipment.
Dependencies are inferred from the claim text.