Regulated vectors for controlling DNA hypermutability in eukaryotic cells
OwnerLuigi Grasso, Nicholas C. Nicolaides +1inventors
InventorsLuigi Grasso, Nicholas C. Nicolaides, Philip M. Sass
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Abstract
The present invention features mammalian expression vectors that are useful for controlling DNA hypermutability in mammalian cell as well as the encoding polynucleotide sequences of vector sequences. In related aspects the invention features expression vectors and host cells comprising such polynucleotides. In other related aspects, the invention features transgenic cells expressing a mutator gene to enhance genome-wide mutagenesis, due to, for example, the presence of an exogenous mutator-encoding polynucleotide sequence. Further, the invention provides methods for using vector sequences that can remove the expression of such gene to restore DNA stability in a host cell.
Claims22
A polynucleotide vector comprising a constitutively active promoter operatively linked to a sequence encoding a dominant negative allele of a mismatch repair gene, an internal ribosome entry site -and a negative selection marker sequence.
Dependencies are inferred from the claim text.