In vivo stimulation of angiogenic activity
AssigneesLudwig Institute for Cancer Research, Licentia Ltd., Aventis Pharma S.A.
InventorsBerndt Enholm, Didier Branellec, Seppo Ylae-Herttuala, Ralf Pettersson, Erika Bergsten, Ulf Eriksson, Kari Alitalo, Aude Le Roux
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Abstract
Angiogenesis is stimulated by introducing two replication-deficient adenovirus vectors to the endothelial cells or proximate to the endothelial cells of an organism. The first vector encodes VEGF-B167 or a fragment or conservative substitution thereof, the second vector encodes VEGF-A or VEGF-C, or fragments or conservative substitutions thereof.
Claims27
A method of stimulating angiogenesis, comprising:
- introducing a first replication-deficient adenovirus vector comprising a polynucleotide sequence encoding VEGF-B167 or a fragment or conservative substitution thereof to an organism;
- and
- introducing a second replication-deficient adenovirus vector comprising a polynucleotide sequence encoding VEGF-A or a fragment or conservative substitution thereof to said organism,
- wherein said first replication-deficient adenovirus vector and said second replication-deficient adenovirus vector are delivered to at least one living cell selected from the group consisting of endothelial cells and cells proximate to endothelial cells of said organism.
A method of stimulating angiogenesis, comprising:
- introducing a first replication-deficient adenovirus vector comprising a polynucleotide sequence encoding VEGF-B167 or a fragment or conservative substitution thereof to an organism;
- and
- introducing a second replication-deficient adenovirus vector comprising a polynucleotide sequence encoding VEGF-C or a fragment or conservative substitution thereof to said organism,
- wherein said first replication-deficient adenovirus vector and said second replication-deficient adenovirus vector are delivered to at least one living cell selected from the group consisting of endothelial cells and cells proximate to endothelial cells of said organism.
A method of stimulating angiogenesis, comprising:
- introducing a first replication-deficient adenovirus vector comprising a polynucleotide sequence encoding VEGF-B167 or a fragment or conservative substitution thereof to an organism;
- and
- introducing a second replication-deficient adenovirus vector comprising a polynucleotide sequence encoding VEGF-D or a fragment or conservative substitution thereof to said organism,
- wherein said first replication-deficient adenovirus vector and said second replication-deficient adenovirus vector are delivered to at least one living cell selected from the group consisting of endothelial cells and cells proximate to endothelial cells of said organism.
Dependencies are inferred from the claim text.