Replication protein A and use
AssigneePioneer Hi-Bred International, Inc.
InventorPramod B. Mahajan
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Abstract
Methods and compositions for modulating DNA metabolism are provided. Nucleotide and amino acid sequences encoding a maize replication protein A subunits are provided. The sequences can be used in expression cassettes for modulating DNA replication, DNA repair, and recombination.
Claims27
An isolated protein comprising a polypeptide having at least 80% sequence identity over the full-length of SEQ ID NOS:
- 2 or 4, wherein the percent sequence identity is determined by the GAP algorithm under default parameters and wherein the polypeptide enhances homologous recombination as part of an RPA complex.
An isolated protein comprising a polypeptide encoded by SEQ ID NOS:
- 1 or 3, wherein the polypeptide enhances homologous recombination as part of an RPA complex.
8 A host cell comprising the expression cassette of claim 7.
An antibody that selectively binds to an isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2 or 4.
An antibody that selectively binds to an isolated polypeptide comprising at least 20 contiguous amino acids of the amino acid sequence selected from the group consisting of SEQ ID NO:
- 2 or 4.
An isolated polypeptide wherein:
- (a) the polypeptide comprises at least 20 contiguous amino acids of a polypeptide selected from the group consisting of SEQ ID NO:
- 2 or 4;
- (b) the polypeptide, when presented as an immunogen, elicits the production of an antibody which specifically binds the polypeptide selected from the group consisting of SEQ ID NO:
- 2 or 4;
- (c) the polypeptide does not bind to antisera raised against the polypeptide of SEQ ID NO:
- 2 or 4 after the antisera has been fully immunosorbed with the polypeptide of SEQ ID NO:
- 2 or 4;
- and
- (d) the polypeptide enhances homologous recombination as part of an RPA complex.
Dependencies are inferred from the claim text.