Heterologous gene expression in plants
OwnerGeert Angenon, Geert De Jaeger +2inventors
InventorsGeert Angenon, Geert De Jaeger, Alain Goossens, Anna Depicker
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Abstract
The present invention relates to heterologous gene expression in plants. More specifically, the invention relates to high expression of heterologous proteins in seeds, by incorporating the gene between seed specific sequences. Preferably, the heterologous protein is a single-chain antibody variable fragment (scFv).
Claims37
A seed preferred expression cassette having gene regulatory elements comprising:
- a) a promoter comprising an arcelin promoter of sequence of SEQ ID NO:
- 1 or a phaseolin promoter of SEQ ID NO:
- 5;
- b) a leader comprising an arcelin 5I leader sequence of SEQ ID NO:
- 2 or a TMV omega leader;
- and
- c) an arcelin 5I 3′ end comprising the sequence of SEQ ID NO:
- 3.
A process for obtaining seed preferred expression of a heterologous protein at a level of at least about 10% of the total soluble seed protein, wherein said heterologous protein is not an unmodified seed storage protein, said process comprising:
- introducing into a plant or plant cell a gene of interest encoding a heterologous protein;
- growing said plant or plant cell;
- and
- expressing a heterologous protein at a level of at least about 10% of the total soluble seed protein, provided that said heterologous protein is not an unmodified seed storage protein.
Dependencies are inferred from the claim text.