Polynucleotides encoding polypeptides having invertase activity and use of same

An isolated nucleic acid comprising a genomic, complementary or composite polynucleotide sequence encoding a polypeptide having an invertase activity, said polypeptide is at least 80% homologous to SEQ ID NO:5 as determined using the BestFit software of the Wisconsin sequence analysis package, utilizing the Smith and Waterman algorithm, where gap creation penalty equals 8 and gap extension penalty equals 2.

An isolated nucleic acid comprising a genomic, complementary or composite polynucleotide sequence hybridizable with SEQ ID NOs:1, 4 or 6 under hybridization, conditions of hybridization solution containing 10% dextrane sulfate, 1 M NaCl, 1% SDS and 5×106 cpm 32p labeled probe, at 65° C., with a final wash solution of 0.1×SSC and 0.1% SDS and final wash at 60° C.

An isolated nucleic acid comprising a genomic, complementary or composite polynucleotide sequence at least 80% identical with SEQ ID NOs:1, 4 or 6 as determined using the BestFit software of the Wisconsin sequence analysis package, utilizing the Smith and Waterman algorithm, where gap weight equals 50, length weight equals 3, average match equals 10 and average mismatch equals −9.

An isolated nucleic acid comprising a polynucleotide sequence as set forth in SEQ ID NOs:1, 4 or 6.

An isolated nucleic acid comprising a polynucleotide sequence encoding a polypeptide as set forth in SEQ ID NO:5.

A recombinant protein comprising a polypeptide having an invertase activity in an apoplastic environment and an N terminal amino acid sequence serving for secretion into an apoplast.

A recombinant protein comprising a polypeptide as set forth in SEQ ID NO:5.

A recombinant protein comprising a polypeptide at least 80% homologous to SEQ ID NO:5 as determined using the BestFit software of the Wisconsin sequence analysis package, utilizing the Smith and Waterman algorithm, where gap creation penalty equals 8 and gap extension penalty equals 2.

A method of increasing a level of a monosaccharide in a plant tissue, the method comprising the step of expressing in the plant tissue a polypeptide having invertase activity, wherein said polypeptide is at least 80% homologous to SEQ ID NO:5 as determined using the BestFit software of the Wisconsin sequence analysis package, utilizing the Smith and Waterman algorithm where gap creation penalty equals 8 and gap extension penalty equals 2.

A method of increasing a level of a monosaccharide in a plant tissue, the method comprising the step of expressing a polypeptide having invertase activity, wherein said polypeptide is encoded by a polynucleotide hybridizable with SEQ ID NOs:1, 4 or 6 or a portion thereof under hybridization conditions of hybridization solution containing 10% dextrane sulfate, 1 M NaCl, 1% SDS and 5×106 cpm 32p labeled probe, at 65° C., with a final wash solution of 0.1×SSC and 0.1% SDS and final wash at 60° C.

A method of increasing a level of a monosaccharide in a plant tissue, the method comprising the step of expressing a polypeptide having invertase activity, wherein said polypeptide is encoded by a polynucleotide at least 80% identical with SEQ ID NO:6 as determined using the BestFit software of the Wisconsin sequence analysis package, utilizing the Smith and Waterman algorithm, where gap weight equals 50, length weight equals 3, average match equals 10 and average mismatch equals −9.