Method and compositions for the production of transgenic plants

A method for producing a genetically-modified oil palm plant, said method comprising:

• obtaining an explant from an oil palm plant;
• culturing said explant in callus-induction medium to generate an embryogenic callus therefrom;
• transforming said embryogenic callus with genetic material to provide a transformed embryogenic callus;
• selecting the transformed embryogenic callus;
• forming a polyembryogenic callus culture from the transformed embryogenic callus;
• and
• regenerating transformed plantlets from said polyembryogenic callus cultures.

A method for producing a genetically-modified oil palm plant comprising:

• obtaining an explant from an oil palm plant;
• culturing said explant in callus-induction medium to generate a Type II embryogenic callus therefrom;
• transforming said Type II embryogenic callus with genetic material to provide a transformed Type II embryogenic callus;
• selecting the transformed Type II embryogenic callus;
• forming a polyembryogenic callus culture from the transformed Type II embryogenic callus;
• and
• regenerating transformed plantlets from said polyembryogenic callus culture.

A method for the transformation and regeneration of an oil palm plant comprising:

• obtaining an immature embryo explant from an oil palm plant;
• transforming said immature embryo explant with genetic material to provide a transformed immature embryo explant;
• culturing said transformed immature embryo explant in callus-induction medium to generate a transformed embryogenic callus;
• selecting said transformed embryogenic callus;
• forming a polyembryogenic callus culture from said transformed embryogenic callus;
• and
• regenerating transformed plantlets from said polyembryogenic callus cultures.