Cell culture system and methods of use
AssigneePurdue Research Foundation
InventorsPaul Collodi, Lianchun Fan, Chunguang Ma
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Abstract
The present invention is directed to methods of making a fish embryo cell line that can give rise to germ cells when introduced to a fish embryo. The present invention is further directed to methods of using the fish embryo cell line, making germ line chimeric fish, and to a cell culture medium.
Claims31
A fish embryo cell line, wherein a cell of the fish embryo cell line, after incubation in vitro for at least about 24 hours, will become a germ cell when introduced to a fish embryo.
A method for making a fish embryo cell line, the method comprising:
- providing an isolated fish cell obtained from a first fish embryo;
- incubating the isolated fish cell in vitro for at least about 24 hours, wherein the fish cell or progeny thereof become a germ cell when introduced to a second fish embryo.
A method for making a germ line chimeric fish, the method comprising:
- providing an isolated fish cell that has been incubated in vitro for at least about 24 hours;
- introducing the fish cell to a recipient fish embryo to result in a chimeric fish embryo;
- and
- incubating the chimeric fish embryo such that the embryo develops into a chimeric fish comprising a germ cell derived from the introduced fish cell.
A cell culture medium comprising a growth factor and fish cell conditioned medium, wherein the growth factor is fibroblast growth factor or epidermal growth factor.
A cell culture medium comprising a growth factor and a fish cell, wherein the growth factor is fibroblast growth factor or epidermal growth factor.
A cell line having the characteristics of a cell line designated ZEB.
A cell line having the characteristics of a cell line designated ZEG.
Dependencies are inferred from the claim text.