Compositions and methods for the synthesis of fatty acids, their derivatives and downstream products
OwnerJohn Joseph Kopchick, Bruce Kelder +3inventors
InventorsJohn Joseph Kopchick, Bruce Kelder, Yung-Sheng Huang, Stephen J. Kirchner, Pradip Mukerji
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Abstract
The invention generally relates to synthesis of essential fatty acids and their derivatives, long-chain polyunsaturated fatty acids (LC-PUFAs) and eicosanoids in transfected cells and in transgenic animals.
Claims31
A composition, comprising animal cells producing essential fatty acids.
A composition, comprising transfected animal cells, said transfected animal cells producing altered levels of long-chain polyunsaturated fatty acids relative to the animal cells prior to transfection.
A method, comprising:
- a) providing:
- i) a non-human animal cell, ii) a vector comprising nucleic acid encoding a heterologous desaturase, and iii) a recipient non-human female animal;
- b) introducing said vector into said cell to create a transfected cell;
- and c) transferring said transfected cell into said recipient female under conditions such that at least one offspring is produced, said offspring expressing said desaturase in one or more tissues.
A transgenic non-human animal, said animal producing at least one product, said product selected from the group consisting of arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid.
Dependencies are inferred from the claim text.